ProGP348 (TfsB (S-layer protein))
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| ProGP ID | ProGP348 (TfsB (S-layer protein)) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Tannerella forsythia ATCC 43037 |
| Domain | Bacteria |
| Classification | Phylum : Bacteroidetes Class : Bacteroidia Orders : Bacteroidales Family : Tanerellaceae Genus : Tannerella Species : forsythia Strain : ATCC 43037 |
| Taxonomic ID (NCBI) | 203275 |
| Genome Information | |
| GenBank | GQ856797 |
| EMBL | GQ856797 |
| Organism Additional Information | Tannerella forsythia is an oral pathogen causing chronic periodontitis. It forms polymicrobial subgingival plaque biofilm along with Porphyromonas gingivalis and Treponema denticola. |
| Gene Information | |
| Gene Name | tfsB (TF2663) |
| Protein Information | |
| Protein Name | TfsB (S-layer protein) |
| UniProtKB/SwissProt ID | A0A1D3UN43 |
| EMBL-CDS | SCQ21562.1. |
| UniProtKB Sequence | >tr|A0A1D3UN43|A0A1D3UN43_TANFO S-layer protein subunit OS=Tannerella forsythia GN=tfsB PE=4 SV=1 MNKKIFTLLAGAFMGFAAVSSVTAQIALEQQQLRVKPYDNAKPTLKFKEGANDGYYYLQV DSMVAYDKVTKRQKMIGFTPKKTPWPGVIPLDSFGTIRRGVTLYMGPDSAGQNALWIDVM GRLSGTTPGPRVPSAYPNTASIALESAAGLWCVTIQKYYQGQNPTFDFTNKLQRMMLEVN VYGHESWPKDVDGWRKSNYKSSSNPNAQTPGSISAWEFSETYKTGVKGGRPLVSYLDETM DTVAVLVGDTLNKLGYVQVKIAPATDVKAGKINGMLLFTLKEAMPFALDKKSFETVLGTQ ASQEARSLKFSPNGTPNPFTDQKWEVEYLDGISPVTAMLIDSSAWTNPWETNYRAWDVAP ISQVGTAGNPWFSVDVRHMGQMGYVRLKNAGKYLYVDTVYYNSGNSQFLKFNQNATFPGD RNPMIGQFVWRMVYYPSGDSIYINPFSATYLPTYDPKAITDSTRFATNSVVRSWYTFTAY PTPKLMADTLPRYNQNGLSHPALQVPVDERMTARLKWGVNPKRKIGAVTPGAGAPIDYSY YHRLYVSLQNLTVDGKPGTRVTLHNTKENGKINTQINFGCYEPCKMATSDRVSIDPDLYL IRNERGQYLHVPLYSAHDSAVWVFLDKNVHPEELPSFQWIVEKRFKNAEASPINIINREF GQINKGGMYAGNYGLAFMDIQLKKDQHAPFKGRTKTWGWNKQIVNSEVIDFEKTKNNMSA KKAPTFIRLPKQYKNNPNLGYQWINRDTATVNLYAFNYRSGIDNTKYLSVKASIFDGTGR DSLIYVQGTNCFDIAYFRIDTVNVDAGHYKPYGYGVKAGAESPSVWKKNQVADLVQLKRQ AYRIKYENPFKYCLGALKLSNGEQYRYSLSSRLRYNEVIGTPLFFLRDVYMEEDGVKDFA LVQIIDTFSIQSTGGGLTPSAQLEKYLKEHLGAQLTSLIMGNLKKAGEFNPGVFVAAVEE QAPMKLKYTYRGDVQTRVSTFRLKRDADPIYRRFNTELEGDKPGDTPETVKFYTTRSAPT GKNYLFENTNALKDQQAYYRAKKNYLGLVSINSNPNVKTAIYVDTAYVNRGTGHIKPQYM LMIRPTIVEDTLGCDDNGDLTIPLPGYRRGMYLINATDSAKADAGTPNENDYLWGTAWNR LVFTDAIHANDALYILGHADLKGLYTKINLKGEKMLDLKKLDAASDSSPAAPKNGKIRKI ALNNNFHKDCVFSFRLIERKSCDKEGGDFLIESETQDRGQYPVIAPCDGGWIKIQNGVPV ISRSDVLQAIADAEIFNVEKTKEDPVANEITPAITEVKVVAENGAVTILNAAGKKVVVSN VLGQTLVNTVLTSDRATVAAPQGVVVVVVEGQPAVKAMVK |
| Sequence length | 1360 AA |
| Function | S-layer helps T. forsythia in adhesion/invasion to human gingival epithelial cells and to epidermal carcinoma cells of the mouth. The host immune response to this pathogen is attenuated by the S-layer after immune evasion. |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S473, T493, T752, T786, T1069 |
| Glycosite(s) Annotated Protein Sequence | >tr|A0A1D3UN43|A0A1D3UN43_TANFO S-layer protein subunit OS=Tannerella forsythia GN=tfsB PE=4 SV=1 MNKKIFTLLAGAFMGFAAVSSVTAQIALEQQQLRVKPYDNAKPTLKFKEGANDGYYYLQV DSMVAYDKVTKRQKMIGFTPKKTPWPGVIPLDSFGTIRRGVTLYMGPDSAGQNALWIDVM GRLSGTTPGPRVPSAYPNTASIALESAAGLWCVTIQKYYQGQNPTFDFTNKLQRMMLEVN VYGHESWPKDVDGWRKSNYKSSSNPNAQTPGSISAWEFSETYKTGVKGGRPLVSYLDETM DTVAVLVGDTLNKLGYVQVKIAPATDVKAGKINGMLLFTLKEAMPFALDKKSFETVLGTQ ASQEARSLKFSPNGTPNPFTDQKWEVEYLDGISPVTAMLIDSSAWTNPWETNYRAWDVAP ISQVGTAGNPWFSVDVRHMGQMGYVRLKNAGKYLYVDTVYYNSGNSQFLKFNQNATFPGD RNPMIGQFVWRMVYYPSGDSIYINPFSATYLPTYDPKAITDSTRFATNSVVRS*(473)WYTFTAY PTPKLMADT*(493)LPRYNQNGLSHPALQVPVDERMTARLKWGVNPKRKIGAVTPGAGAPIDYSY YHRLYVSLQNLTVDGKPGTRVTLHNTKENGKINTQINFGCYEPCKMATSDRVSIDPDLYL IRNERGQYLHVPLYSAHDSAVWVFLDKNVHPEELPSFQWIVEKRFKNAEASPINIINREF GQINKGGMYAGNYGLAFMDIQLKKDQHAPFKGRTKTWGWNKQIVNSEVIDFEKTKNNMSA KKAPTFIRLPKQYKNNPNLGYQWINRDTAT*(786)VNLYAFNYRSGIDNTKYLSVKASIFDGTGR DSLIYVQGTNCFDIAYFRIDTVNVDAGHYKPYGYGVKAGAESPSVWKKNQVADLVQLKRQ AYRIKYENPFKYCLGALKLSNGEQYRYSLSSRLRYNEVIGTPLFFLRDVYMEEDGVKDFA LVQIIDTFSIQSTGGGLTPSAQLEKYLKEHLGAQLTSLIMGNLKKAGEFNPGVFVAAVEE QAPMKLKYTYRGDVQTRVSTFRLKRDADPIYRRFNTELEGDKPGDTPETVKFYTTRSAPT GKNYLFENTNALKDQQAYYRAKKNYLGLVSINSNPNVKTAIYVDTAYVNRGT*(1069)GHIKPQYM LMIRPTIVEDTLGCDDNGDLTIPLPGYRRGMYLINATDSAKADAGTPNENDYLWGTAWNR LVFTDAIHANDALYILGHADLKGLYTKINLKGEKMLDLKKLDAASDSSPAAPKNGKIRKI ALNNNFHKDCVFSFRLIERKSCDKEGGDFLIESETQDRGQYPVIAPCDGGWIKIQNGVPV ISRSDVLQAIADAEIFNVEKTKEDPVANEITPAITEVKVVAENGAVTILNAAGKKVVVSN VLGQTLVNTVLTSDRATVAAPQGVVVVVVEGQPAVKAMVK |
| Sequence Around Glycosites (21 AA) | TTRFATNSVVRSWYTFTAYPTP AYPTPKLMADTLPRYNQNGLS GYQWINRDTATVNLYAFNYRS GRDSLIYVQGTNCFDIAYFR YVDTAYVNRGTGHIKPQYMLM |
| Technique(s) used for Glycosylation Detection | Pro-Q Emerald carbohydrate staining |
| Protein Glycosylation- Implication | Here, the protein O-glycosylation system is needed for creating a rich glycoproteome that is possibly related to the virulence of this bacterium. |
| Glycan Information | |
| Glycan Annotation | An oligosaccharide with the structure 4-Me-β-ManpNAcCONH2-(1→3)-[Pse5Am7Gc-(2→4)-]-β-ManpNAcA-(1→4)-[4-Me-α-Galp-(1→2)-]-α-Fucp-(1→4)-[-α-Xylp-(1→3)-]-β-GlcpA-(1→3)-[-β-Digp-(1→2)-]-α-Galp. |
| GlyTouCan | G58135UN |
| Protein Glycosylation linked (PGL) gene(s) | |
| Additional Comment | Until now, T. Forsythia is the only known Gram-negative bacterium that is covered with a glycosylated S-layer, with, again uniquely, two S-layer glycoproteins being simultaneously present. |
| Literature | |
| Year of Identification | 2011 |
| Year of Identification Month Wise | 2011.2.4 |
| Year of Validation | 2011 |
| Reference | Posch, G., Pabst, M., Brecker, L., Altmann, F., Messner, P. and Schäffer, C., 2011. Characterization and scope of S-layer protein O-glycosylation in Tannerella forsythia. Journal of Biological Chemistry, 286(44), pp.38714-38724. |
| Corresponding Author | Christina Schaffer |
| Contact | Department of NanoBiotechnology, NanoGlycobiology, Vienna Institute of BioTechnology, Universität für Bodenkultur Wien, Muthgasse 11, A-1190 Vienna, Austria. |