ProGP348 (TfsB (S-layer protein))

Home -> ProGPdb -> Search ProGP -> Display data

ProGP ID ProGP348 (TfsB (S-layer protein))
Validation Status Characterized
Organism Information
Organism NameTannerella forsythia ATCC 43037
Domain Bacteria
Classification Phylum : Bacteroidetes
Class : Bacteroidia
Orders : Bacteroidales
Family : Tanerellaceae
Genus : Tannerella
Species : forsythia
Strain : ATCC 43037
Taxonomic ID (NCBI) 203275
Genome Information
GenBank GQ856797
EMBL GQ856797
Organism Additional Information Tannerella forsythia is an oral pathogen causing chronic periodontitis. It forms polymicrobial subgingival plaque biofilm along with Porphyromonas gingivalis and Treponema denticola.
Gene Information
Gene NametfsB (TF2663)
Protein Information
Protein NameTfsB (S-layer protein)
UniProtKB/SwissProt ID A0A1D3UN43
EMBL-CDSSCQ21562.1.
UniProtKB Sequence >tr|A0A1D3UN43|A0A1D3UN43_TANFO S-layer protein subunit OS=Tannerella forsythia GN=tfsB PE=4 SV=1 MNKKIFTLLAGAFMGFAAVSSVTAQIALEQQQLRVKPYDNAKPTLKFKEGANDGYYYLQV DSMVAYDKVTKRQKMIGFTPKKTPWPGVIPLDSFGTIRRGVTLYMGPDSAGQNALWIDVM GRLSGTTPGPRVPSAYPNTASIALESAAGLWCVTIQKYYQGQNPTFDFTNKLQRMMLEVN VYGHESWPKDVDGWRKSNYKSSSNPNAQTPGSISAWEFSETYKTGVKGGRPLVSYLDETM DTVAVLVGDTLNKLGYVQVKIAPATDVKAGKINGMLLFTLKEAMPFALDKKSFETVLGTQ ASQEARSLKFSPNGTPNPFTDQKWEVEYLDGISPVTAMLIDSSAWTNPWETNYRAWDVAP ISQVGTAGNPWFSVDVRHMGQMGYVRLKNAGKYLYVDTVYYNSGNSQFLKFNQNATFPGD RNPMIGQFVWRMVYYPSGDSIYINPFSATYLPTYDPKAITDSTRFATNSVVRSWYTFTAY PTPKLMADTLPRYNQNGLSHPALQVPVDERMTARLKWGVNPKRKIGAVTPGAGAPIDYSY YHRLYVSLQNLTVDGKPGTRVTLHNTKENGKINTQINFGCYEPCKMATSDRVSIDPDLYL IRNERGQYLHVPLYSAHDSAVWVFLDKNVHPEELPSFQWIVEKRFKNAEASPINIINREF GQINKGGMYAGNYGLAFMDIQLKKDQHAPFKGRTKTWGWNKQIVNSEVIDFEKTKNNMSA KKAPTFIRLPKQYKNNPNLGYQWINRDTATVNLYAFNYRSGIDNTKYLSVKASIFDGTGR DSLIYVQGTNCFDIAYFRIDTVNVDAGHYKPYGYGVKAGAESPSVWKKNQVADLVQLKRQ AYRIKYENPFKYCLGALKLSNGEQYRYSLSSRLRYNEVIGTPLFFLRDVYMEEDGVKDFA LVQIIDTFSIQSTGGGLTPSAQLEKYLKEHLGAQLTSLIMGNLKKAGEFNPGVFVAAVEE QAPMKLKYTYRGDVQTRVSTFRLKRDADPIYRRFNTELEGDKPGDTPETVKFYTTRSAPT GKNYLFENTNALKDQQAYYRAKKNYLGLVSINSNPNVKTAIYVDTAYVNRGTGHIKPQYM LMIRPTIVEDTLGCDDNGDLTIPLPGYRRGMYLINATDSAKADAGTPNENDYLWGTAWNR LVFTDAIHANDALYILGHADLKGLYTKINLKGEKMLDLKKLDAASDSSPAAPKNGKIRKI ALNNNFHKDCVFSFRLIERKSCDKEGGDFLIESETQDRGQYPVIAPCDGGWIKIQNGVPV ISRSDVLQAIADAEIFNVEKTKEDPVANEITPAITEVKVVAENGAVTILNAAGKKVVVSN VLGQTLVNTVLTSDRATVAAPQGVVVVVVEGQPAVKAMVK
Sequence length 1360 AA
Function S-layer helps T. forsythia in adhesion/invasion to human gingival epithelial cells and to epidermal carcinoma cells of the mouth. The host immune response to this pathogen is attenuated by the S-layer after immune evasion.
Glycosylation Status
Glycosylation Type O- (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS473, T493, T752, T786, T1069
Glycosite(s) Annotated Protein Sequence >tr|A0A1D3UN43|A0A1D3UN43_TANFO S-layer protein subunit OS=Tannerella forsythia GN=tfsB PE=4 SV=1 MNKKIFTLLAGAFMGFAAVSSVTAQIALEQQQLRVKPYDNAKPTLKFKEGANDGYYYLQV DSMVAYDKVTKRQKMIGFTPKKTPWPGVIPLDSFGTIRRGVTLYMGPDSAGQNALWIDVM GRLSGTTPGPRVPSAYPNTASIALESAAGLWCVTIQKYYQGQNPTFDFTNKLQRMMLEVN VYGHESWPKDVDGWRKSNYKSSSNPNAQTPGSISAWEFSETYKTGVKGGRPLVSYLDETM DTVAVLVGDTLNKLGYVQVKIAPATDVKAGKINGMLLFTLKEAMPFALDKKSFETVLGTQ ASQEARSLKFSPNGTPNPFTDQKWEVEYLDGISPVTAMLIDSSAWTNPWETNYRAWDVAP ISQVGTAGNPWFSVDVRHMGQMGYVRLKNAGKYLYVDTVYYNSGNSQFLKFNQNATFPGD RNPMIGQFVWRMVYYPSGDSIYINPFSATYLPTYDPKAITDSTRFATNSVVRS*(473)WYTFTAY PTPKLMADT*(493)LPRYNQNGLSHPALQVPVDERMTARLKWGVNPKRKIGAVTPGAGAPIDYSY YHRLYVSLQNLTVDGKPGTRVTLHNTKENGKINTQINFGCYEPCKMATSDRVSIDPDLYL IRNERGQYLHVPLYSAHDSAVWVFLDKNVHPEELPSFQWIVEKRFKNAEASPINIINREF GQINKGGMYAGNYGLAFMDIQLKKDQHAPFKGRTKTWGWNKQIVNSEVIDFEKTKNNMSA KKAPTFIRLPKQYKNNPNLGYQWINRDTAT*(786)VNLYAFNYRSGIDNTKYLSVKASIFDGTGR DSLIYVQGTNCFDIAYFRIDTVNVDAGHYKPYGYGVKAGAESPSVWKKNQVADLVQLKRQ AYRIKYENPFKYCLGALKLSNGEQYRYSLSSRLRYNEVIGTPLFFLRDVYMEEDGVKDFA LVQIIDTFSIQSTGGGLTPSAQLEKYLKEHLGAQLTSLIMGNLKKAGEFNPGVFVAAVEE QAPMKLKYTYRGDVQTRVSTFRLKRDADPIYRRFNTELEGDKPGDTPETVKFYTTRSAPT GKNYLFENTNALKDQQAYYRAKKNYLGLVSINSNPNVKTAIYVDTAYVNRGT*(1069)GHIKPQYM LMIRPTIVEDTLGCDDNGDLTIPLPGYRRGMYLINATDSAKADAGTPNENDYLWGTAWNR LVFTDAIHANDALYILGHADLKGLYTKINLKGEKMLDLKKLDAASDSSPAAPKNGKIRKI ALNNNFHKDCVFSFRLIERKSCDKEGGDFLIESETQDRGQYPVIAPCDGGWIKIQNGVPV ISRSDVLQAIADAEIFNVEKTKEDPVANEITPAITEVKVVAENGAVTILNAAGKKVVVSN VLGQTLVNTVLTSDRATVAAPQGVVVVVVEGQPAVKAMVK
Sequence Around Glycosites (21 AA) TTRFATNSVVRSWYTFTAYPTP
AYPTPKLMADTLPRYNQNGLS
GYQWINRDTATVNLYAFNYRS
GRDSLIYVQGTNCFDIAYFR
YVDTAYVNRGTGHIKPQYMLM
Technique(s) used for Glycosylation DetectionPro-Q Emerald carbohydrate staining
Protein Glycosylation- Implication Here, the protein O-glycosylation system is needed for creating a rich glycoproteome that is possibly related to the virulence of this bacterium.
Glycan Information
Glycan Annotation An oligosaccharide with the structure 4-Me-β-ManpNAcCONH2-(1→3)-[Pse5Am7Gc-(2→4)-]-β-ManpNAcA-(1→4)-[4-Me-α-Galp-(1→2)-]-α-Fucp-(1→4)-[-α-Xylp-(1→3)-]-β-GlcpA-(1→3)-[-β-Digp-(1→2)-]-α-Galp.
GlyTouCan G58135UN
Protein Glycosylation linked (PGL) gene(s)
Additional CommentUntil now, T. Forsythia is the only known Gram-negative bacterium that is covered with a glycosylated S-layer, with, again uniquely, two S-layer glycoproteins being simultaneously present.
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.4
Year of Validation 2011
ReferencePosch, G., Pabst, M., Brecker, L., Altmann, F., Messner, P. and Schäffer, C., 2011. Characterization and scope of S-layer protein O-glycosylation in Tannerella forsythia. Journal of Biological Chemistry, 286(44), pp.38714-38724.
Corresponding Author Christina Schaffer
ContactDepartment of NanoBiotechnology, NanoGlycobiology, Vienna Institute of BioTechnology, Universität für Bodenkultur Wien, Muthgasse 11, A-1190 Vienna, Austria.