ProGP351 (BF0810)

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ProGP ID ProGP351 (BF0810)
Validation Status Characterized
Organism Information
Organism NameBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Domain Bacteria
Classification Family: Bacteroidaceae
Order: "Bacteroidales"
Class: "Bacteroidia"
Division or phylum: "Bacteroidetes"
Taxonomic ID (NCBI) 272559
Genome Information
GenBank CR626927.1
EMBL CR626927
Organism Additional Information The Bacteroides constitute the major population of human intestinal microbiota. They are beneficial to the humans in terms of metabolism, development, and immunity. They play roles in recycling of bile acids, provision of short-chain fatty acids to the host and angiogenesis.
Gene Information
Gene NameBF0810
NCBI Gene ID 3288800
GenBank Gene Sequence NC_003228.3
Protein Information
Protein NameBF0810
UniProtKB/SwissProt ID Q5LH32
NCBI RefSeq YP_210506.1
EMBL-CDSCAH06554.1
UniProtKB Sequence >tr|Q5LH32|Q5LH32_BACFN Putative alpha-L-fucosidase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF0810 PE=4 SV=1 MKNNRLIITLIALFLLGFGLKAQTASTEETAAQKEKRMEWFAQAKLGIFIHWGIYAVNGV SESWSFFNNYLPYEEYMAQEKGFTASAYNPQEWVKLIKESGARYTVITTKHHDGVALWDT KAGDLSTVKSTPAGRDLIAPFVKEVRKQGLKLGFYYSLLDWSHPDYPNKTRTEVRYKNDP DRWAKFVKFNFGQLSELNKTWKPDLYWFDGDWEQTAEAWDSKGIINLLRSTNPNVIVNSR IQGYGDYATPEQGVPVVRPADKYWELCMTMNDSWGYQHADTNYKTPFMLLRTFVDCLSMG GNLLLDIGPKEDGTIPAEQIAVLKEFGRWTKKHKEAIYETRAGIPCEHFQGYTTLNKAGD ILYLYLPYKPNGPIEVKGLVNKVNRVWVVGNGAMLPYKVYNKNYWSEVPGNLYIDIPERV QDEQITVIAVLLDGPIKLYRGVGQVIESN
Sequence length 449 AA
Function Putative alpha-L-fucosidase.
Glycosylation Status
Glycosylation Type O- (Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinT84, T281 (Engineered glycosylation site DTA was created at two positions F83D and N282A)
Experimentally Validated Glycosite(s ) in Mature ProteinT84, T281 (Engineered glycosylation site DTA was created at two positions F83D and N282A)
Glycosite(s) Annotated Protein Sequence >tr|Q5LH32|Q5LH32_BACFN Putative alpha-L-fucosidase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF0810 PE=4 SV=1 MKNNRLIITLIALFLLGFGLKAQTASTEETAAQKEKRMEWFAQAKLGIFIHWGIYAVNGV SESWSFFNNYLPYEEYMAQEKGDT*(84)ASAYNPQEWVKLIKESGARYTVITTKHHDGVALWDT KAGDLSTVKSTPAGRDLIAPFVKEVRKQGLKLGFYYSLLDWSHPDYPNKTRTEVRYKNDP DRWAKFVKFNFGQLSELNKTWKPDLYWFDGDWEQTAEAWDSKGIINLLRSTNPNVIVNSR IQGYGDYATPEQGVPVVRPADKYWELCMTMNDSWGYQHADT*(281)AYKTPFMLLRTFVDCLSMG GNLLLDIGPKEDGTIPAEQIAVLKEFGRWTKKHKEAIYETRAGIPCEHFQGYTTLNKAGD ILYLYLPYKPNGPIEVKGLVNKVNRVWVVGNGAMLPYKVYNKNYWSEVPGNLYIDIPERV QDEQITVIAVLLDGPIKLYRGVGQVIESN
Sequence Around Glycosites (21 AA) EEYMAQEKGFTASAYNPQEWV
NDSWGYQHADTNYKTPFMLLR
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Technique(s) used for Glycosylation DetectionCoomassie staining, Pro-Q Emerald glycostaining, and reactivity with anti-glycan serum
Technique(s) used for Glycosylated Residue(s) Detection Known residues are mutated to create glycosylation motif/sequon
Glycan Information
Glycan Annotation Exogenous fucose.
Technique(s) used for Glycan Identification Lectin (AAL)binding
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.4
Year of Validation 2011
ReferenceFletcher, C.M., Coyne, M.J. and Comstock, L.E., 2011. Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. Journal of Biological Chemistry, 286(5), pp.3219-3226.
Corresponding Author Laurie E. Comstock
ContactChanning Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
ReferenceFletcher, C.M., Coyne, M.J. and Comstock, L.E. (2011) Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. J Biol Chem, 286, 3219-3226. [PubMed: 21115495]
Author Fletcher, C.M., Coyne, M.J. and Comstock, L.E.
Research GroupChanning Laboratory, Brigham and Womens Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Corresponding Author Comstock, L.E.
ContactChanning Laboratory, Brigham and Womens Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.