ProGP398 (Lectin LecB)
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| ProGP ID | ProGP398 (Lectin LecB) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Pseudomonas aeruginosa PAO1 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : gammaproteobacteria Orders : Pseudomonadales Family : Pseudomonadaceae Genus : Pseudomonas Species : aeruginosa Strain : PAO1 |
| Taxonomic ID (NCBI) | 1147787 |
| Genome Information | |
| GenBank | NC_002516.2 |
| EMBL | AE004091 |
| Organism Additional Information | This Gram-negative opportunistic pathogen produces LecA and LecB virulence factors at high levels. It is involved in chronic airway infections. These virulence factors have been demonstrated to play a role in P. aeruginosa-induced otitis externa diffusa and cystic fibrosis. |
| Gene Information | |
| Gene Name | lecB (PA3361) |
| NCBI Gene ID | 882528 |
| GenBank Gene Sequence | NC_002516.2 |
| Protein Information | |
| Protein Name | Lectin LecB (PA-IIL is old name) |
| UniProtKB/SwissProt ID | Q9HYN5 |
| NCBI RefSeq | NP_252051.1 |
| EMBL-CDS | AAG06749.1 |
| UniProtKB Sequence | >tr|Q9HYN5|Q9HYN5_PSEAE Fucose-binding lectin PA-IIL OS=Pseudomonas aeruginosa GN=lecB PE=1 SV=1 MATQGVFTLPANTRFGVTAFANSSGTQTVNVLVNNETAATFSGQSTNNAVIGTQVLNSGS SGKVQVQVSVNGRPSDLVSAQVILTNELNFALVGSEDGTDNDYNDAVVVINWPLG |
| Sequence length | 115 AA |
| Subcellular Location | Periplasm or outer membrane |
| Function | LecB is a fucose-/mannose-specific lectin. It may help in the adhesion of P. aeruginosa cells to the receptors and facilitate biofilm formation resulting in host tissue colonization. This virulence factor has an important role in human infection. It inhibits the ciliary beating and hence defence mechanism of the lung. It is involved in cystic fibrosis. |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N22 |
| Experimentally Validated Glycosite(s ) in Mature Protein | N22 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q9HYN5|Q9HYN5_PSEAE Fucose-binding lectin PA-IIL OS=Pseudomonas aeruginosa GN=lecB PE=1 SV=1 MATQGVFTLPANTRFGVTAFAN*(22)SSGTQTVNVLVNNETAATFSGQSTNNAVIGTQVLNSGS SGKVQVQVSVNGRPSDLVSAQVILTNELNFALVGSEDGTDNDYNDAVVVINWPLG |
| Sequence Around Glycosites (21 AA) | NTRFGVTAFANSSGTQTVNVL |
| Technique(s) used for Glycosylation Detection | Deglycosylation with N-glycosidase F (PNGase F) |
| Technique(s) used for Glycosylated Residue(s) Detection | Site-directed mutagenesis (N22A) |
| Protein Glycosylation- Implication | LecB N glycosylation seems to be important for its production and transport to the outer membrane. |
| Protein Glycosylation linked (PGL) gene(s) | |
| Additional Comment | A LecB-deficient P. aeruginosa strain has been shown to be impaired in biofilm formation. |
| Literature | |
| Year of Identification | 2011 |
| Year of Identification Month Wise | 2011.2.18 |
| Year of Validation | 2011 |
| Reference | Bartels, K.M., Funken, H., Knapp, A., Brocker, M., Bott, M., Wilhelm, S., Jaeger, K.E. and Rosenau, F., 2011. Glycosylation is required for outer membrane localization of the lectin LecB in Pseudomonas aeruginosa. Journal of bacteriology, 193(5), pp.1107-1113. |
| Corresponding Author | Frank Rosenau |
| Contact | Institute of Microbiology and Biotechnology, Ulm University, Ulm, Germany. |