ProGP412 (OmpA/MotB)
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| ProGP ID | ProGP412 (OmpA/MotB) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Acinetobacter baumannii ATCC 17978 |
| Domain | Bacteria |
| Classification | Family: Moraxellaceae Order: Pseudomonadales Class: Gammaproteobacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 400667 |
| Genome Information | |
| GenBank | CP000521.1 |
| EMBL | CP018664.1 |
| Organism Additional Information | Acinetobacter baumannii is an opportunistic nosocomial pathogen infecting immunocompromised patients. It causes pneumonia, septicaemia, urinary tract infections and meningitis. There has been a rise in the multidrug-resistant as well as pandrug-resistant strains of the ?superbug?. |
| Gene Information | |
| Gene Name | A1S_1193 |
| GenBank Gene Sequence | CP000521.1 |
| Protein Information | |
| Protein Name | OmpA/MotB |
| NCBI RefSeq | ABO11623 |
| EMBL-CDS | ABO11623.1 |
| UniProtKB Sequence | >ABO11623.2 OmpA/MotB [Acinetobacter baumannii ATCC 17978] MSINPIELLKEKVSSTILNNQDGYLGEKTNALSKFYPILLSLLAAKPDLIGQLKNSLAPSLSDLFSHNEQ IKNTVLTHLSGTAPNNEIENTLNSALKPSLNAISDVAGNDQQSIVNYLRQHAETIRSYLPGWAVGLLAPL GIGAGLSSVTSSTAPPLAAATETTGKSRGFLPIIALIILGLLIAWLWRSCQHKEATPAPETKAASGVEAA AAPATLTLSTDDKGAVSQCQAGIGDQGFLATLQTQVKQVFSATKDCDVDTSQTYAAAFTDKDALAGVLGA LKGIPNASLEWVGDKITLKAGDAAALEALTAKVKALVPHTEVVAAAPETAEQSVSNSLSASQTALTAIDP NNVDVNALVKALNLQIINFASGSSDIPADNKAILDQAATLLNKVSGVKLDVGGHTDSTGNAAANKALSQR RAQAVVDYLVSKGVDASKLVAKGHGSEQPVADNTTEEGRFKNRRIEFSVAQ |
| Sequence length | 471 AA |
| Subcellular Location | Outer Membrane |
| Function | Shows homology with peptidoglycan binding and outer membrane stabilizing proteins |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S205 |
| Glycosite(s) Annotated Protein Sequence | >ABO11623.2 OmpA/MotB [Acinetobacter baumannii ATCC 17978] MSINPIELLKEKVSSTILNNQDGYLGEKTNALSKFYPILLSLLAAKPDLIGQLKNSLAPSLSDLFSHNEQ IKNTVLTHLSGTAPNNEIENTLNSALKPSLNAISDVAGNDQQSIVNYLRQHAETIRSYLPGWAVGLLAPL GIGAGLSSVTSSTAPPLAAATETTGKSRGFLPIIALIILGLLIAWLWRSCQHKEATPAPETKAAS*(205)GVEAA AAPATLTLSTDDKGAVSQCQAGIGDQGFLATLQTQVKQVFSATKDCDVDTSQTYAAAFTDKDALAGVLGA LKGIPNASLEWVGDKITLKAGDAAALEALTAKVKALVPHTEVVAAAPETAEQSVSNSLSASQTALTAIDP NNVDVNALVKALNLQIINFASGSSDIPADNKAILDQAATLLNKVSGVKLDVGGHTDSTGNAAANKALSQR RAQAVVDYLVSKGVDASKLVAKGHGSEQPVADNTTEEGRFKNRRIEFSVAQ |
| Sequence Around Glycosites (21 AA) | ATPAPETKAASGVEAAAAPAT |
| Technique(s) used for Glycosylation Detection | ZIC HILIC and Mass spectrometry (LTQ-Orbitrap Velos) |
| Technique(s) used for Glycosylated Residue(s) Detection | GluC/trypsin cocktail digestion, HILIC-enrichment and LC-MS/UVPD |
| Glycan Information | |
| Glycan Annotation | Pentasaccharide β-GlcNAc3NAcA4OAc-4-(β-GlcNAc-6-)-α-Gal-6-β-Glc-3-β-GalNAc-S/T. β-GlcNAc3NAcA4OAcA is an O-acetylated derivative of glucuronic acid. |
| BCSDB ID | 29567 |
| GlyTouCan | G82631BC |
| Technique(s) used for Glycan Identification | 1H:13C HSQC 2D NMR and mass spectrometry |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | PglLAb |
| OST ProGT ID | ProGT55 (PglLAb) |
| Literature | |
| Reference | Iwashkiw, J.A., Seper, A., Weber, B.S., Scott, N.E., Vinogradov, E., Stratilo, C., Reiz, B., Cordwell, S.J., Whittal, R., Schild, S. and Feldman, M.F., 2012. Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation. PLoS pathogens, 8(6), p.e1002758. |
| Corresponding Author | Mario F Feldman |
| Contact | Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada |
| Reference | Iwashkiw JA, Seper A, Weber BS, Scott NE, Vinogradov E, Stratilo C, Reiz B, Cordwell SJ, Whittal R, Schild S, Feldman MF. (2012) Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation. PLoS Pathog., 8(6):e1002758. [PubMed: 22685409] |
| Author | Iwashkiw JA, Seper A, Weber BS, Scott NE, Vinogradov E, Stratilo C, Reiz B, Cordwell SJ, Whittal R, Schild S, Feldman MF. |
| Research Group | Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada |
| Corresponding Author | Feldman MF. |
| Contact | Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada |