ProGP42 (Cellobiosidase)
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| ProGP ID | ProGP42 (Cellobiosidase) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Fibrobacter (Bacteroides) succinogenes S85 |
| Domain | Bacteria |
| Classification | Family: "Fibrobacteraceae" Order: "Fibrobacterales" Class: "Fibrobacteria" Division or phylum: "Fibrobacteres" |
| Taxonomic ID (NCBI) | 59374 |
| Genome Information | |
| GenBank | CP001792.1 |
| EMBL | CP001792 |
| Gene Information | |
| Gene Name | Fisuc_2992 |
| NCBI Gene ID | 8523639 |
| GenBank Gene Sequence | NC_013410.1 |
| Protein Information | |
| Protein Name | Cellobiosidase |
| UniProtKB/SwissProt ID | A7UG54 |
| NCBI RefSeq | YP_003251054.1 |
| EMBL-CDS | ACX76572.1 |
| UniProtKB Sequence | >tr|A7UG54|A7UG54_FIBSS Chloride-stimulated cellobiosidase OS=Fibrobacter succinogenes (strain ATCC 19169 / S85) GN=Fisuc_2992 PE=3 SV=1 MNFKKNKAFAIAATVAMALSVPSFAQLNNGDMEYGDGGWYLWNNPDGPAVAESQIAVQGL GVDGSQGAKVVVKELPNPWWGLQLQPPKFLADSGFYKLSFKAKGNMPINSVVQGGPPDYR QKESASFDLTDKWQTYEMIFLADQKGYGLNNITFQIGLKKGWIQLDDVEVEKMDEMSDPS WYNNADARIDSLRKVDFTVKANPGEKVHVKLLRHSFPFGTALALYDTKDSTENWYRNAAK KYFWHGVSENQFKWPEYEPKKGKIKRDEMKEYTDFTAQNHWKLRGHALMWSHQGYGFDKH YSNKGRCEEMAEKLKARIYRDLKEYKGKITEYDVWNEPIHESWTFNKCGWGILDSAFIWA HKADPSAFLYINDYNVVAAGETDRYYGLIKGMLERKVPVMGIGVQCHFGLRPVVPSLIKE RLDKLASLGLPIKVTEFDVGDWQAGMNDTEEVQAEKFETFLRTAFSHPAVNGIVFWGFWD NRHWVKNGGMIASDGREKPAAKRVYDLWHKVWTTDLYATADENGEAKFRGFKGYYQVNAG DKKFQITVK |
| Sequence length | 549 AA |
| Subcellular Location | Extracellular as well as periplasmic |
| Function | Extracellular cellobiosidase acitivity is stimulated by chloride. Cl-stimulated cellobiosidase has also been found to be associated with cell membrane debris. It is capable of hydrolysing p-nitrophenyl lactoside and p-nitrophenyl cellobioside. A detectable activity on carboxymethyl cellulose has also been found. |
| Glycosylation Status | |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining |
| Glycan Information | |
| Glycan Annotation | Carbohydrate content between 8 and 16% (mainly cellohexaose). |
| Literature | |
| Year of Identification | 1988 |
| Year of Identification Month Wise | 1988.07 |
| Reference | Huang, L., Forsberg, C.W. and Thomas, D.Y., 1988. Purification and characterization of a chloride-stimulated cellobiosidase from Bacteroides succinogenes S85. Journal of bacteriology, 170(7), pp.2923-2932. |
| Corresponding Author | C W Forsberg |
| Contact | Department of Microbiology, University of Guelph, Ontario, Canada. |
| Reference | Huang, L., Forsberg, C.W. and Thomas, D.Y. (1988) Purification and characterization of a chloride-stimulated cellobiosidase from Bacteroides succinogenes S85. J Bacteriol, 170, 2923-2932. [PubMed: 3384799] |
| Author | Thomas, D.Y. |
| Research Group | Department of Microbiology, University of Guelph, Ontario, Canada. |
| Corresponding Author | Huang, L., Forsberg, C.W. Thomas, D.Y. |
| Contact | Department of Microbiology, University of Guelph, Ontario, Canada. |