ProGP422 (LafA (Lateral flagellin))
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| ProGP ID | ProGP422 (LafA (Lateral flagellin)) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Aeromonas hydrophila AH-3 (serotype O34) |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : gammaproteobacteria Orders : Aeromonadales Family : Aeromonadaceae Genus : Aeromonas Species : hydrophila Strain : AH-3 |
| Taxonomic ID (NCBI) | 644 |
| Genome Information | |
| GenBank | NZ_MTPO01000007.1. |
| EMBL | DQ860510 |
| Gene Information | |
| Gene Name | lafA |
| GenBank Gene Sequence | NZ_LYZG01000004.1 |
| Protein Information | |
| Protein Name | LafA (Lateral flagellin) |
| UniProtKB/SwissProt ID | C5E7P9 |
| NCBI RefSeq | WP_029302047.1 |
| UniProtKB Sequence | >tr|C5E7P9|C5E7P9_AERHY Flagellin OS=Aeromonas hydrophila GN=lafA1 PE=3 SV=1 MGLSIHTNFASLTTQSQLNTTNKMLGTAMQRLGTGLRINSAADDAAGLQMATRLQSQSSG QKVGMRNSQDAISMMQTGEGAMDEMSNIVQRMKDLATQSANGTSTTEDRKAMDAEFTELR AELDNISNNTTFGGQELLKTGKGFQEDVTFQIGGTGAEKLELKKTGTLAKALAEVVGGGK GTDGAAVKAGIGAQDKATAAMAELDTFSQKIGESRSAFGANINRLEHTVNNLSNMKENTD LANGRIMDADFAQESTNMTKNQMLMQAGMSVLSNSNQMTGMVTSLLR |
| Sequence length | 287 AA |
| Subcellular Location | Flagellum |
| Function | Lateral flagellin is inducible. |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S178 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q9AET1|Q9AET1_AERHY Flagellin OS=Aeromonas hydrophila GN=lafA PE=3 SV=1 MGLSIHTNFASMTTQNQLNSTNKMLGTAMQRLGTGLRINSAADDAAGLQIATRLQAQSNG QKVGMRNAQDAISMIQTAESAMDEMTNITQRMKDLATQAANGTNSTKDLDAMDAEFKELG KELGNIRDNTTFGGTSLLKTGGKFQATGGVTFQIGASVDEKLVFDASTKVTSVNTAIS*(178)TA SAAALTTGAAAQITAMDSMLEKIGEARSAFGANINRLEHTVNNLSNMKENTDMANGRIMD ADFAQESTNMTKNQMLMQAGMSVLSNSNQMTGMVTGLLR |
| Sequence Around Glycosites (21 AA) | TKVTSVNTAISTASAAALTTG |
| Protein Glycosylation- Implication | O-Glycosylation is necessary for flagellar biogenesis. Pseudaminic acid biosynthetic genes pseB and pseF homologues, were found to be required for flagellar formation and flagellin expression. |
| Glycan Information | |
| Glycan Annotation | A 376 Da monosaccharide which is pseudaminic acid (Pse5Ac7Ac) modified by the addition of an acetyl group and water. This sugar was detected at three sites in the protein sequence. |
| Literature | |
| Year of Identification | 2012 |
| Year of Identification Month Wise | 2012.8.10 |
| Year of Validation | 2012 |
| Reference | Wilhelms, M., Fulton, K.M., Twine, S.M., Tomás, J.M. and Merino, S., 2012. Differential glycosylation of polar and lateral flagellins in Aeromonas hydrophila AH-3. Journal of Biological Chemistry, 287(33), pp.27851-27862. |
| Corresponding Author | Juan M. Tomás |
| Contact | Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain |