ProGP422 (LafA (Lateral flagellin))
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ProGP ID | ProGP422 (LafA (Lateral flagellin)) |
Validation Status | Characterized |
Organism Information | |
Organism Name | Aeromonas hydrophila AH-3 (serotype O34) |
Domain | Bacteria |
Classification | Family: Aeromonadaceae Order: Aeromonadales Class: Gammaproteobacteria Division or phylum: "Proteobacteria" |
Taxonomic ID (NCBI) | 644 |
Genome Information | |
GenBank | NZ_MTPO01000007.1. |
EMBL | DQ860510 |
Gene Information | |
Gene Name | lafA |
GenBank Gene Sequence | NZ_LYZG01000004.1 |
Protein Information | |
Protein Name | LafA (Lateral flagellin) |
UniProtKB/SwissProt ID | C5E7P9 |
NCBI RefSeq | WP_029302047.1 |
UniProtKB Sequence | >tr|C5E7P9|C5E7P9_AERHY Flagellin OS=Aeromonas hydrophila GN=lafA1 PE=3 SV=1 MGLSIHTNFASLTTQSQLNTTNKMLGTAMQRLGTGLRINSAADDAAGLQMATRLQSQSSG QKVGMRNSQDAISMMQTGEGAMDEMSNIVQRMKDLATQSANGTSTTEDRKAMDAEFTELR AELDNISNNTTFGGQELLKTGKGFQEDVTFQIGGTGAEKLELKKTGTLAKALAEVVGGGK GTDGAAVKAGIGAQDKATAAMAELDTFSQKIGESRSAFGANINRLEHTVNNLSNMKENTD LANGRIMDADFAQESTNMTKNQMLMQAGMSVLSNSNQMTGMVTSLLR |
Sequence length | 287 AA |
Subcellular Location | Flaggellum |
Function | Lateral flagellin is inducible. |
Glycosylation Status | |
Glycosylation Type | O- (Ser) linked |
Experimentally Validated Glycosite(s) in Full Length Protein | S178 |
Glycosite(s) Annotated Protein Sequence | >tr|Q9AET1|Q9AET1_AERHY Flagellin OS=Aeromonas hydrophila GN=lafA PE=3 SV=1 MGLSIHTNFASMTTQNQLNSTNKMLGTAMQRLGTGLRINSAADDAAGLQIATRLQAQSNG QKVGMRNAQDAISMIQTAESAMDEMTNITQRMKDLATQAANGTNSTKDLDAMDAEFKELG KELGNIRDNTTFGGTSLLKTGGKFQATGGVTFQIGASVDEKLVFDASTKVTSVNTAIS*(178)TA SAAALTTGAAAQITAMDSMLEKIGEARSAFGANINRLEHTVNNLSNMKENTDMANGRIMD ADFAQESTNMTKNQMLMQAGMSVLSNSNQMTGMVTGLLR |
Sequence Around Glycosites (21 AA) | TKVTSVNTAISTASAAALTTG |
Protein Glycosylation- Implication | O-Glycosylation is necessary for flagellar biogenesis. Pseudaminic acid biosynthetic genes pseB and pseF homologues, were found to be required for flagellar formation and flagellin expression. |
Glycan Information | |
Glycan Annotation | A 376 Da monosaccharide which is pseudaminic acid (Pse5Ac7Ac) modified by the addition of an acetyl group and water. This sugar was detected at three sites in the protein sequence. |
Literature | |
Year of Identification | 2012 |
Year of Identification Month Wise | 2012.8.10 |
Year of Validation | 2012 |
Reference | Wilhelms M, Fulton KM, Twine SM, Tomás JM, Merino S. (2012) Differential glycosylation of polar and lateral flagellins in Aeromonas hydrophila AH-3. J Biol Chem. 287(33):27851-62. [PMID: 22733809] |
Author | Wilhelms M, Fulton KM, Twine SM, Tomás JM, Merino S |
Research Group | Department of Microbiology, Faculty of Biology, University of Barcelona, ??Diagonal 645, 08071 Barcelona, ??Spain. |
Corresponding Author | Merino S |
Contact | Department of Microbiology, Faculty of Biology, University of Barcelona, ??Diagonal 645, 08071 Barcelona, ??Spain. |