
Technique(s) used for Glycosylation Detection | Migration on SDS-PAGE , a lectin blot using GlcNAc-specific succinylated wheat germ agglutinin (sWGA), Glycoprotein enrichment with agarose bound WGA |
Technique(s) used for Glycosylated Residue(s) Detection | MALDI-TOF/TOF, LC-MS/MS analysis |
Glycan Information |
Glycan Annotation | Monosaccharide (Double HexNAc, HexNAc) |
Protein Glycosylation linked (PGL) gene(s) |
OST ProGT ID | |
Literature |
Reference | Rolain, T., Bernard, E., Beaussart, A., Degand, H., Courtin, P., Egge-Jacobsen, W., Bron, P.A., Morsomme, P., Kleerebezem, M., Chapot-Chartier, M.P. and Dufrêne, Y.F., 2013. O-glycosylation as a novel control mechanism of peptidoglycan hydrolase activity. Journal of Biological Chemistry, 288(31), pp.22233-22247. |
Corresponding Author | Pascal Hols |
Contact | Institute of Life Sciences, Catholic University of Louvain, B-1348 Louvain-la-Neuve, Belgium |
Reference | Fredriksen, L., Moen, A., Adzhubei, A.A., Mathiesen, G., Eijsink, V.G. and Egge-Jacobsen, W., 2013. Lactobacillus plantarum WCFS1 O-linked protein glycosylation: an extended spectrum of target proteins and modification sites detected by mass spectrometry. Glycobiology, 23(12), pp.1439-1451. |
Corresponding Author | Wolfgang Egge-Jacobsen |
Contact | NORBRAIN Mass Spectrometry Facility, Unit for Genome Dynamics, Department of Microbiology, Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway |
Reference | Rolain T, Bernard E, Beaussart A, Degand H, Courtin P, Egge-Jacobsen W, Bron PA, Morsomme P, Kleerebezem M, Chapot-Chartier MP, Dufrêne YF, Hols P. (2013) O-glycosylation as a novel control mechanism of peptidoglycan hydrolase activity. J Biol Chem., 288(31):22233-47. [PubMed: 23760506] |
Author | Rolain T, Bernard E, Beaussart A, Degand H, Courtin P, Egge-Jacobsen W, Bron PA, Morsomme P, Kleerebezem M, Chapot-Chartier MP, Dufrêne YF, Hols P |
Research Group | Institute of Life Sciences, Catholic University of Louvain, B-1348 Louvain-la-Neuve, Belgium |
Corresponding Author | Hols P |
Contact | Institute of Life Sciences, Catholic University of Louvain, B-1348 Louvain-la-Neuve, Belgium |
Reference | Fredriksen L, Moen A, Adzhubei AA, Mathiesen G, Eijsink VG, Egge-Jacobsen W. (2013) Lactobacillus plantarum WCFS1 O-linked protein glycosylation: an extended spectrum of target proteins and modification sites detected by mass spectrometry. Glycobiology, 23(12), 1439-51. [PubMed: 24000282] |
Author | Fredriksen L, Moen A, Adzhubei AA, Mathiesen G, Eijsink VG, Egge-Jacobsen W |
Research Group | Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432 Aas, Norway. |
Corresponding Author | Egge-Jacobsen W |
Contact | Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432 Aas, Norway. |