ProGP437 (FliC (Type B Flagellin))

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ProGP ID ProGP437 (FliC (Type B Flagellin))
Validation Status Characterized
Organism Information
Organism NamePseudomonas aeruginosa strain UCBPP-PA14
Domain Bacteria
Classification Phylum : Proteobacteria
Class : gammaproteobacteria
Orders : Pseudomonadales
Family : Pseudomonadaceae
Genus : Pseudomonas
Species : aeruginosa
Strain : UCBPP-PA14
Taxonomic ID (NCBI) 208963
Genome Information
GenBank NC_008463.1.
EMBL CP000438
Organism Additional Information Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen responsible for nosocomial pneumonia. It possesses a multitude of virulence factors including type IV pili that mediate adhesion to host cells. It is also the major cause of mortality among cystic fibrosis (CF) patients.
Gene Information
Gene NamefliC
Protein Information
Protein NameFliC (Type B Flagellin)
UniProtKB/SwissProt ID A0A0H2Z7X1
NCBI RefSeq WP_003082185.1
EMBL-CDSABJ10257.1
UniProtKB Sequence >tr|A0A0H2Z7X1|A0A0H2Z7X1_PSEAB Flagellin OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=fliC PE=3 SV=1 MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG LNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG TVASVAGTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT SATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTVVTGYVQLNSP TAYSVSGTGTQASQVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADLG AVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQLP QAVLSLLR
Sequence length 484 AA
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN26, N69 and N439
Glycosite(s) Annotated Protein Sequence >tr|A0A0H2Z7X1|A0A0H2Z7X1_PSEAB Flagellin OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=fliC PE=3 SV=1 MALTVNTNIASLNTQRNLNASSNDLN*(26)TSLQRLTTGYRINSAKDDAAGLQISNRLSNQISG LNVATRNAN*(69)DGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQ AELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAG TVASVAGTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVF TADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTIT SATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTVVTGYVQLNSP TAYSVSGTGTQASQVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADLG AVQNRFKNTIDNLTNISEN*(439)ATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQLP QAVLSLLR
Sequence Around Glycosites (21 AA) RNLNASSNDLNTSLQRLTTGY
SGLNVATRNANDGISLAQTAE
TIDNLTNISENATNARSRIKD
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Technique(s) used for Glycosylation Detectionconcanavalin A and wheat germ agglutinin
Technique(s) used for Glycosylated Residue(s) Detection NanoLC MS
Protein Glycosylation linked (PGL) gene(s)
Additional CommentFlagellin FliC is shown to exhibit glycopolymorphism (with both N- and O- glycoforms present) probably resulting from macroheterogeneity and microheterogeneity.
Literature
Year of Identification2013
Year of Identification Month Wise2013.1
Year of Validation 2013
ReferenceKhemiri, A., Naudin, B., Franck, X., Chan Tchi Song, P., Jouenne, T. and Cosette, P., 2013. N-glycosidase treatment with 18O labeling and de novo sequencing argues for flagellin FliC glycopolymorphism in Pseudomonas aeruginosa. Analytical and bioanalytical chemistry, 405(30), pp.9835-9842.
Corresponding Author Pascal Cosette
ContactCNRS, UMR 6270, Laboratory "Polymères, Biopolymères, Surfaces", 76820, Mont-Saint-Aignan, France