ProGP439 (Lp_2260)
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| ProGP ID | ProGP439 (Lp_2260) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Lactobacillus plantarum |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Lactobacillales Family : Lactobacillaceae Genus : Lactobacillus Species : plantarum |
| Taxonomic ID (NCBI) | 220668 |
| Genome Information | |
| GenBank | NC_004567.2. |
| EMBL | AL935263 |
| Gene Information | |
| NCBI Gene ID | 1062579 |
| GenBank Gene Sequence | NC_004567.2. |
| Protein Information | |
| Protein Name | Lp_2260 |
| UniProtKB/SwissProt ID | F9UQH9 |
| NCBI RefSeq | WP_003641538.1. |
| EMBL-CDS | CCC79468.1 |
| UniProtKB Sequence | >tr|F9UQH9|F9UQH9_LACPL Extracellular protein, DUF336 family OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) GN=lp_2260 PE=4 SV=1 MSKKGFLLGVVLGGAAIATAVMKMDDDKKAELKAKAQQGIADFKDRAIDYAFYANDAAED FKAEAGQQFEDTKRKVADFADQYQAAKQETGESFGSSLDQATDSLRSELAKVEDDEGDDE DIVIDGGAAYQKAGEDEKTSAPVPESAPAKDATPAKSDDESTTDESSPAK |
| Sequence length | 170 AA |
| Subcellular Location | Extracellular |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S146 |
| Glycosite(s) Annotated Protein Sequence | >tr|F9UQH9|F9UQH9_LACPL Extracellular protein, DUF336 family OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) GN=lp_2260 PE=4 SV=1 MSKKGFLLGVVLGGAAIATAVMKMDDDKKAELKAKAQQGIADFKDRAIDYAFYANDAAED FKAEAGQQFEDTKRKVADFADQYQAAKQETGESFGSSLDQATDSLRSELAKVEDDEGDDE DIVIDGGAAYQKAGEDEKTS*(146)APVPESAPAKDATPAKSDDESTTDESSPAK |
| Sequence Around Glycosites (21 AA) | DEKTSAPVPESAPAKDATPAK |
| Technique(s) used for Glycosylation Detection | Glycoprotein enrichment with agarose bound WGA |
| Technique(s) used for Glycosylated Residue(s) Detection | LC-MS/MS analysis |
| Glycan Information | |
| Glycan Annotation | Monosaccharide (Double HexNAc, HexNAc) |
| Literature | |
| Year of Identification | 2013 |
| Year of Identification Month Wise | 2013.12.23 |
| Reference | Fredriksen, L., Moen, A., Adzhubei, A.A., Mathiesen, G., Eijsink, V.G. and Egge-Jacobsen, W., 2013. Lactobacillus plantarum WCFS1 O-linked protein glycosylation: an extended spectrum of target proteins and modification sites detected by mass spectrometry. Glycobiology, 23(12), pp.1439-1451. |
| Corresponding Author | Wolfgang Egge-Jacobsen |
| Contact | NORBRAIN Mass Spectrometry Facility, Unit for Genome Dynamics, Department of Microbiology, Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway |