ProGP468 (C-terminal protease)
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| ProGP ID | ProGP468 (C-terminal protease) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Burkholderia cenocepacia K56-2 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Betaproteobacteria Orders : Burkholderiales Family : Burkholderiaceae Genus : Burkholderia Species : cenocepacia Strain : K56 |
| Taxonomic ID (NCBI) | 985075 |
| Genome Information | |
| GenBank | NZ_LAUA01000000 |
| EMBL | LAUA01000000 |
| Organism Additional Information | Burkholderia cenocepacia causes opportunistic infections in plants, insects, animals and humans. It acts as a health threat to cystic fibrosis patients, causing lethal necrotizing pneumonia in some cases. |
| Gene Information | |
| Gene Name | bcal0738 |
| GenBank Gene Sequence | NC_011000.1 |
| Protein Information | |
| Protein Name | C-terminal protease |
| UniProtKB/SwissProt ID | B4EA63 |
| NCBI RefSeq | CAR51046 |
| UniProtKB Sequence | >tr|B4EA63|B4EA63_BURCJ C-terminal processing protease-3 OS=Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) GN=BCAL0738 PE=3 SV=1 MRMNLKNIGLIAAGLATGVFATLQVSASAEQTATAPLPLDQLRLFAEVFGQIKREYVEPV DDKKLLTAAIKGMVSSLDPHSSFLDKTDYDELQEQTKGRFAGLGIEISQEDGLVKVISPI EDTPAFRAGIRPGDLITRINDKPVRGMTLDKAVKQMRGEPGTKVTLTIFRKSDDRTFPVT VTRAIIKVQSVKMKMLDPGYAYVRITSFQERTTPDLAQKLQDIARQQPNLKGLILDLRNN GGGLLQSAVGVAGAFLPPDSVVVSTNGQIPDSKQVYRDTYDNYRLPSFDGDPLKNLPPIF KTVPMIVLTNAYSASASEIVAGALQDSKRAQIMGKTTFGKGSVQTVRPMTADTALRLTTA YYYTPSGRSIQNKGITPDVPVDQYADGDPDDVLVTREVDYTNHLANTQDPNEKKEQEERE QRRMDQLRILEEQNDKKTPEQRQKDRDRKPIEFGSADDFMMQQALNKLEGKPVQESKSLL AESTTKSPAAKAATASKASGASAAKPASAPKPASAPK |
| Sequence length | 517 AA |
| Subcellular Location | Periplasm |
| Function | Serine type peptidase activity, serine residue in proteolytic active site. |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S506 and S512 |
| Glycosite(s) Annotated Protein Sequence | >tr|B4EA63|B4EA63_BURCJ C-terminal processing protease-3 OS=Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) GN=BCAL0738 PE=3 SV=1 MRMNLKNIGLIAAGLATGVFATLQVSASAEQTATAPLPLDQLRLFAEVFGQIKREYVEPV DDKKLLTAAIKGMVSSLDPHSSFLDKTDYDELQEQTKGRFAGLGIEISQEDGLVKVISPI EDTPAFRAGIRPGDLITRINDKPVRGMTLDKAVKQMRGEPGTKVTLTIFRKSDDRTFPVT VTRAIIKVQSVKMKMLDPGYAYVRITSFQERTTPDLAQKLQDIARQQPNLKGLILDLRNN GGGLLQSAVGVAGAFLPPDSVVVSTNGQIPDSKQVYRDTYDNYRLPSFDGDPLKNLPPIF KTVPMIVLTNAYSASASEIVAGALQDSKRAQIMGKTTFGKGSVQTVRPMTADTALRLTTA YYYTPSGRSIQNKGITPDVPVDQYADGDPDDVLVTREVDYTNHLANTQDPNEKKEQEERE QRRMDQLRILEEQNDKKTPEQRQKDRDRKPIEFGSADDFMMQQALNKLEGKPVQESKSLL AESTTKSPAAKAATASKASGASAAKPAS*(506)APKPAS*(512)APK |
| Sequence Around Glycosites (21 AA) | ASGASAAKPASAPKPASAPK AKPASAPKPASAPK |
| Technique(s) used for Glycosylation Detection | ZIC-HILIC, immunoblotting, tryptic digestion, and MS/MS analysis |
| Technique(s) used for Glycosylated Residue(s) Detection | MS/MS analysis |
| Glycan Information | |
| Glycan Annotation | Trisaccharide HexNAc-HexNAc-Hex. |
| BCSDB ID | 12058 |
| GlyTouCan | G71937MV |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | PglLBC |
| OST ProGT ID | ProGT71 |
| Literature | |
| Year of Identification | 2014 |
| Year of Identification Month Wise | 2014.3.5 |
| Reference | Lithgow, K.V., Scott, N.E., Iwashkiw, J.A., Thomson, E.L., Foster, L.J., Feldman, M.F. and Dennis, J.J., 2014. A general protein O‐glycosylation system within the B urkholderia cepacia complex is involved in motility and virulence. Molecular microbiology, 92(1), pp.116-137. |
| Corresponding Author | Jonathan J. Dennis |
| Contact | Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, |