ProGP471 (Putative uncharacterized protein)
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| ProGP ID | ProGP471 (Putative uncharacterized protein) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Burkholderia cenocepacia K56-2 |
| Domain | Bacteria |
| Classification | Family: Burkholderiaceae Order: Burkholderiales Class: Betaproteobacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 985075 |
| Genome Information | |
| GenBank | NZ_LAUA01000000 |
| EMBL | LAUA01000000 |
| Organism Additional Information | Burkholderia cenocepacia causes opportunistic infections in plants, insects, animals and humans. It acts as a health threat to cystic fibrosis patients, causing lethal necrotizing pneumonia in some cases. |
| Gene Information | |
| Gene Name | bcam2680 |
| GenBank Gene Sequence | NC_011000.1. |
| Protein Information | |
| Protein Name | Putative uncharacterized protein |
| UniProtKB/SwissProt ID | B4EKU9 |
| NCBI RefSeq | WP_012493607.1 |
| EMBL-CDS | CAR56544.1. |
| UniProtKB Sequence | >tr|B4EKU9|B4EKU9_BURCJ Putative membrane protein OS=Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) GN=BCAM2680 PE=4 SV=1 MASADKETVPSTLHALGGVARSRRTRRIGIGVLIFVVLFGLLGFFAAPPLIRHVAEQQLS KQLDRPATIQRIALNPYTLNLEADGIHLGERGGQGDFIDIGKLVVRPSWSSLFRGAPIVN EVRLDSPRFHIVRYDAQRFNFTDLIEKFSSPTPKPDSKPTQFSVSNIQVNDGRIDFDDRL LNEKHVVDNWTLGIPYIATLASKTDIFVEPKLRARFDGSPISIDGKTKPFAQSRESEIAL KFDRLDVPKLISYVPAKLPVAVTSGLLSSNLTVNFVMSGETPALRVSGTVDLNDAKVTDR ASAPLFAARGVHVAAAGLEPLRNAMHFDEIRLDQPVIDLSRDKQGVLNVEKLVAQPAAAP KVEAGAGKPAASAVAAASGASGAVAAASGAKAERSAAAKEAPPLDLTIRHVAIDGGTINV DDRVPATPTAMSLTKLAATLDGFSLQGKTPAKYTLSTSLSRGGDVTAEGAFNLAAKQADT KLTVAALALPALQPYLGEATRARVLDGTLGATVNAKTDWGKTPLAAEVADSTVSLKSLKI AAPDNKAPAIVLPDASAKIAKVDVAARTAEIASVDASGLALDVKRLKDGKIDLAALAEPA QAAVPKRTVARKAQAAAPSWHYRIDALNVKEASANFTDLSTPRPVKLAIKPLELSVQKVS DDLTKPLPVQLKATLNRKGSLNVTGDVTAQPLKLGLKINGNRLDAAAFEPYFGSALNATI ASALLNAQGNLTFAQVKDTPRATYRGDVALVDVRMLDKATSDPFAGWRSLALSNLKANYD EKGTDVDAARVTFSNFYGRVLLDAQGRLNLKDVVAKETGPAQSLTRDASKGEPVPLSPGV TPPAAAQAASAAAAQQASVPAAASATVVVKAAPQPQNPVRMHFGELVLQNGRVTYTDNFI KPNYTANLVAIKGTVGAFGTDSTTSAPVDVAANLAGNGPISIKGSVNPLIEKPALDLTAT AHDIELTNLTPYSAKYAGYPITKGKLNVDLHYQLANDQLSANNHIFIDQLTFGDHVENDT ATKLPVKLAISLLKNTRGQIDVNLPVSGSLSNPEFSVGGLIWHAVLNLIAKAVTSPFTLL ANAFGGGGGEDLGYVEFAPGSYALTDTQQKKLDTVVKMLTEKPSIRLDLIGRVDPAQDTP GLRDAYVERLVRQQKLKDVVGQGESVDPMSVKVDKAEYSKYLTRAYKAADFKKPRNLVGL QKTLPDADMKQALADHAPADDNALRALAQQRAQAVRQYLDGKIDSSRMFVVAPKLDAKGI SDKGATTRVDFGLQ |
| Sequence length | 1274 AA |
| Subcellular Location | Inner membrane |
| Function | DUF748, homology to type I phosphodiesterase/nucleotide pyrophosphatase. |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S396 |
| Glycosite(s) Annotated Protein Sequence | >tr|B4EKU9|B4EKU9_BURCJ Putative membrane protein OS=Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) GN=BCAM2680 PE=4 SV=1 MASADKETVPSTLHALGGVARSRRTRRIGIGVLIFVVLFGLLGFFAAPPLIRHVAEQQLS KQLDRPATIQRIALNPYTLNLEADGIHLGERGGQGDFIDIGKLVVRPSWSSLFRGAPIVN EVRLDSPRFHIVRYDAQRFNFTDLIEKFSSPTPKPDSKPTQFSVSNIQVNDGRIDFDDRL LNEKHVVDNWTLGIPYIATLASKTDIFVEPKLRARFDGSPISIDGKTKPFAQSRESEIAL KFDRLDVPKLISYVPAKLPVAVTSGLLSSNLTVNFVMSGETPALRVSGTVDLNDAKVTDR ASAPLFAARGVHVAAAGLEPLRNAMHFDEIRLDQPVIDLSRDKQGVLNVEKLVAQPAAAP KVEAGAGKPAASAVAAASGASGAVAAASGAKAERS*(396)AAAKEAPPLDLTIRHVAIDGGTINV DDRVPATPTAMSLTKLAATLDGFSLQGKTPAKYTLSTSLSRGGDVTAEGAFNLAAKQADT KLTVAALALPALQPYLGEATRARVLDGTLGATVNAKTDWGKTPLAAEVADSTVSLKSLKI AAPDNKAPAIVLPDASAKIAKVDVAARTAEIASVDASGLALDVKRLKDGKIDLAALAEPA QAAVPKRTVARKAQAAAPSWHYRIDALNVKEASANFTDLSTPRPVKLAIKPLELSVQKVS DDLTKPLPVQLKATLNRKGSLNVTGDVTAQPLKLGLKINGNRLDAAAFEPYFGSALNATI ASALLNAQGNLTFAQVKDTPRATYRGDVALVDVRMLDKATSDPFAGWRSLALSNLKANYD EKGTDVDAARVTFSNFYGRVLLDAQGRLNLKDVVAKETGPAQSLTRDASKGEPVPLSPGV TPPAAAQAASAAAAQQASVPAAASATVVVKAAPQPQNPVRMHFGELVLQNGRVTYTDNFI KPNYTANLVAIKGTVGAFGTDSTTSAPVDVAANLAGNGPISIKGSVNPLIEKPALDLTAT AHDIELTNLTPYSAKYAGYPITKGKLNVDLHYQLANDQLSANNHIFIDQLTFGDHVENDT ATKLPVKLAISLLKNTRGQIDVNLPVSGSLSNPEFSVGGLIWHAVLNLIAKAVTSPFTLL ANAFGGGGGEDLGYVEFAPGSYALTDTQQKKLDTVVKMLTEKPSIRLDLIGRVDPAQDTP GLRDAYVERLVRQQKLKDVVGQGESVDPMSVKVDKAEYSKYLTRAYKAADFKKPRNLVGL QKTLPDADMKQALADHAPADDNALRALAQQRAQAVRQYLDGKIDSSRMFVVAPKLDAKGI SDKGATTRVDFGLQ |
| Sequence Around Glycosites (21 AA) | AAASGAKAERSAAAKEAPPLD |
| Technique(s) used for Glycosylation Detection | ZIC-HILIC,immunoblotting,tryptic digestion, and MS/MS analysis |
| Technique(s) used for Glycosylated Residue(s) Detection | MS/MS analysis |
| Glycan Information | |
| Glycan Annotation | Trisaccharide HexNAc-HexNAc-Hex. |
| BCSDB ID | 12058 |
| GlyTouCan | G71937MV |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | PglLBC |
| OST ProGT ID | ProGT71 (PglLBc) |
| Literature | |
| Reference | Lithgow, K.V., Scott, N.E., Iwashkiw, J.A., Thomson, E.L., Foster, L.J., Feldman, M.F. and Dennis, J.J., 2014. A general protein O‐glycosylation system within the B urkholderia cepacia complex is involved in motility and virulence. Molecular microbiology, 92(1), pp.116-137. |
| Corresponding Author | Jonathan J. Dennis |
| Contact | Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, |
| Reference | Lithgow KV, Scott NE, Iwashkiw JA, Thomson EL, Foster LJ, Feldman MF, Dennis JJ. (2014) A general protein O-glycosylation system within the Burkholderia cepacia complex is involved in motility and virulence. Mol Microbiol., 92(1):116-37. [PubMed: 24673753] |
| Author | Lithgow KV, Scott NE, Iwashkiw JA, Thomson EL, Foster LJ, Feldman MF, Dennis JJ. |
| Research Group | Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, |
| Corresponding Author | Dennis JJ. |
| Contact | Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada. |