ProGP496 (PliA)
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| ProGP ID | ProGP496 (PliA) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Acinetobacter nosocomialis |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Gammaproteobacteria Orders : Pseudomonadales Family : Moraxellaceae Genus : Acinetobacter Species : nosocomialis |
| Taxonomic ID (NCBI) | 106654 |
| Genome Information | |
| GenBank | NZ_KB849570.1. |
| EMBL | APPP01000010 |
| Organism Additional Information | Acinetobacter nosocomialis is a medically relevant strain causing bacteremic nosocomial pneumonia. |
| Protein Information | |
| Protein Name | PliA |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S136 |
| Technique(s) used for Glycosylation Detection | Western blot analysis |
| Technique(s) used for Glycosylated Residue(s) Detection | Western blot analysis of whole cell extracts probing for PilAM2 expression and electrophoretic mobility on S132A and S136A mutant, MS/MS analysis |
| Glycan Information | |
| Glycan Annotation | Tetrasaccharide containing (HexNAc)2, Hexose and N-acetyl-deoxyHexose. |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST ProGT ID | ProGT85 |
| Literature | |
| Year of Identification | 2015 |
| Year of Identification Month Wise | 2015.4.6 |
| Year of Validation | 2015 |
| Reference | Harding, C.M., Nasr, M.A., Kinsella, R.L., Scott, N.E., Foster, L.J., Weber, B.S., Fiester, S.E., Actis, L.A., Tracy, E.N., Munson Jr, R.S. and Feldman, M.F., 2015. A cinetobacter strains carry two functional oligosaccharyltransferases, one devoted exclusively to type IV pilin, and the other one dedicated to O‐glycosylation of multiple proteins. Molecular microbiology, 96(5), pp.1023-1041. |
| Corresponding Author | Mario F Feldman |
| Contact | Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2G2, Canada. |