| Technique(s) used for Glycosylation Detection | Biotin-hydrazide labeling after periodate oxidation, sialic acid-specific lectin LFA (limax flavus agglutinin)-binding, mass excess (approx. 6 kDa) detected by intact mass analysis using ESMS (electrospray mass spectrometry) |
| Technique(s) used for Glycosylated Residue(s) Detection | Edman sequencing |
| Protein Glycosylation- Implication | Sone level of glycosylation is necessary for filament assembly. Modification with either Pse5Ac7Ac or PseAm is sufficient for filament assembly.Modification with pseudaminic acid and derivatives is essential for targeting and/or secretion of flagellin. Glycosylation may be required for interaction of the subunits with the flagellin chaperone, FliS, with other components of the flagellar structure or in subunit interactions. |
| Glycan Information |
| Glycan Annotation | More Pse5Ac7Ac (5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid) in this case than from 81-176. Acetamidino and N-methylacetimidoyl derivatives of legionaminic acid, 315 Da Leg5Am7Ac (5-acetamidino-7-acetamido-3,5,7,9,-tetradeoxy-D-glycero-D-galacto-nonulosonic acid) and Leg5AmNMe7Ac (5-E/Z-N-(N-methylacetimidoyl)-7-acetamidino-3,5,7,9-tetradeoxy-D-glycero-D-galacto-nonulosonic), are also present. Leg5Ac7Ac (Leg) is legionaminic acid and Pse5Ac7Ac (Pse) is pseudaminic acid. |
| BCSDB ID | 21877 |
| GlyTouCan | G07086CL |
| Technique(s) used for Glycan Identification | Homo- and heteronuclear correlated two-dimensional 1H NMR, 13C HSQC (heteronuclear single quantum coherence), HMBC (heteronuclear multiple bond coherence), COSY (correlated spectroscopy), TOCSY (total correlation spectroscopy), and NOESY (nuclear Overhauser effect spectroscopy). |
| Literature |
| Year of Identification | 1989 |
| Year of Identification Month Wise | 1989.06 |
| Year of Validation | 1989 |
| Reference | Logan, S.M., Hui, J.P., Vinogradov, E., Aubry, A.J., Melanson, J.E., Kelly, J.F., Nothaft, H. and Soo, E.C., 2009. Identification of novel carbohydrate modifications on Campylobacter jejuni 11168 flagellin using metabolomics‐based approaches. The FEBS Journal, 276(4), pp.1014-1023. |
| Corresponding Author | Patricia Guerry |
| Contact | Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910, USA. |
| Reference | McNally, D.J., Aubry, A.J., Hui, J.P., Khieu, N.H., Whitfield, D., Ewing, C.P., Guerry, P., Brisson, J.R., Logan, S.M. and Soo, E.C., 2007. Targeted metabolomics analysis of Campylobacter coli VC167 reveals legionaminic acid derivatives as novel flagellar glycans. Journal of Biological Chemistry, 282(19), pp.14463-14475. |
| Corresponding Author | Susan M Logan
Evelyn C. Soo
|
| Contact | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada.
National Research Council, Institute for Marine Biosciences, Halifax, Nova Scotia B3H 3Z1, Canada
|
| Reference | Goon, S., Kelly, J.F., Logan, S.M., Ewing, C.P. and Guerry, P., 2003. Pseudaminic acid, the major modification on Campylobacter flagellin, is synthesized via the Cj1293 gene. Molecular microbiology, 50(2), pp.659-671. |
| Corresponding Author | Patricia Guerry |
| Contact | Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910, USA. |
| Reference | Logan, S.M., Kelly, J.F., Thibault, P., Ewing, C.P. and Guerry, P., 2002. Structural heterogeneity of carbohydrate modifications affects serospecificity of Campylobacter flagellins. Molecular microbiology, 46(2), pp.587-597. |
| Corresponding Author | Patricia Guerry |
| Contact | Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910, USA. |
| Reference | Thibault, P., Logan, S.M., Kelly, J.F., Brisson, J.R., Ewing, C.P. and Guerry, P., 2001. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin. Journal of Biological Chemistry, 276(37), pp.34862-34870. |
| Corresponding Author | Susan M Logan |
| Contact | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada. |
| Reference | Doig, P., Kinsella, N., Guerry, P. and Trust, T.J., 1996. Characterization of a post‐translational modification of Campylobacter flagellin: identification of a sero‐specific glycosyl moiety. Molecular microbiology, 19(2), pp.379-387. |
| Corresponding Author | Trevor J. Trust |
| Contact | Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada. |
| Reference | Logan, S.M., Trust, T.J. and Guerry, P., 1989. Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. Journal of bacteriology, 171(6), pp.3031-3038. |
| Corresponding Author | Patricia Guerry |
| Contact | Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910, USA. |
| Reference | Guerry, P., Doig, P., Alm, R.A., Burr, D.H., Kinsella, N. and Trust, T.J., 1996. Identification and characterization of genes required for post‐translational modification of Campylobacter coli VC167 flagellin. Molecular microbiology, 19(2), pp.369-378. |
| Corresponding Author | Patricia Guerry |
| Contact | Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave., Silver Spring, MD 20910, USA. |
| Reference | Logan, S.M., Hui, J.P., Vinogradov, E., Aubry, A.J., Melanson, J.E., Kelly, J.F., Nothaft, H. and Soo, E.C. (2009) Identification of novel carbohydrate modifications on Campylobacter jejuni 11168 flagellin using metabolomics-based approaches. FEBS J, 276, 1014-1023. [PMID: 19154343] |
| Author | Logan, S.M., Hui, J.P., Vinogradov, E., Aubry, A.J., Melanson, J.E., Kelly, J.F., Nothaft, H. and Soo, E.C. |
| Research Group | NRC-Institute for Biological Sciences, Ottawa, Canada. |
| Corresponding Author | Soo, E.C. |
| Contact | NRC-Institute for Biological Sciences, Ottawa, Canada. |
| Reference | McNally, D.J., Aubry, A.J., Hui, J.P., Khieu, N.H., Whitfield, D., Ewing, C.P., Guerry, P., Brisson, J.R., Logan, S.M. and Soo, E.C. (2007) Targeted metabolomics analysis of Campylobacter coli VC167 reveals legionaminic acid derivatives as novel flagellar glycans. J Biol Chem, 282, 14463-14475. [PubMed: 17371878] |
| Author | McNally, D.J., Aubry, A.J., Hui, J.P., Khieu, N.H., Whitfield, D., Ewing, C.P., Guerry, P., Brisson, J.R., Logan, S.M. and Soo, E.C. |
| Research Group | National Research Council, Institute for Biological Sciences, Ottawa, Ontario, Canada. |
| Corresponding Author | Soo, E.C. |
| Contact | National Research Council, Institute for Biological Sciences, Ottawa, Ontario, Canada. |
| Reference | Goon, S., Kelly, J.F., Logan, S.M., Ewing, C.P. and Guerry, P. (2003) Pseudaminic acid, the major modification on Campylobacter flagellin, is synthesized via the Cj1293 gene. Mol Microbiol, 50, 659-671. [PubMed: 14617187] |
| Author | Goon, S., Kelly, J.F., Logan, S.M., Ewing, C.P. Guerry, P. |
| Research Group | Enteric Diseases Department, Naval Medical Research Center, Silver Spring, MD 20910, USA. |
| Corresponding Author | Guerry, P. |
| Contact | Enteric Diseases Department, Naval Medical Research Center, Silver Spring, MD 20910, USA. |
| Reference | Logan, S.M., Kelly, J.F., Thibault, P., Ewing, C.P. and Guerry, P. (2002) Structural heterogeneity of carbohydrate modifications affects serospecificity of Campylobacter flagellins. Mol Microbiol, 46, 587-597. [PubMed: 12406231] |
| Author | Logan, S.M., Kelly, J.F., Thibault, P., Ewing, C.P. Guerry, P. |
| Research Group | London School of Hygiene and Tropical Medicine, |
| Corresponding Author | Guerry, P. |
| Contact | London School of Hygiene and Tropical Medicine, |
| Reference | Thibault, P., Logan, S.M., Kelly, J.F., Brisson, J.R., Ewing, C.P., Trust, T.J. and Guerry, P. (2001) Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin. J Biol Chem, 276, 34862-34870. [PubMed: 11461915] |
| Author | Thibault, P., Logan, S.M., Kelly, J.F., Brisson, J.R., Ewing, C.P., Trust, T.J. Guerry, P. |
| Research Group | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada. |
| Corresponding Author | Guerry, P. |
| Contact | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6, Canada. |
| Reference | Doig, P., Kinsella, N., Guerry, P. and Trust, T.J. (1996) Characterization of a post-translational modification of Campylobacter flagellin: identification of a sero-specific glycosyl moiety. Mol Microbiol, 19, 379-387. [PubMed: 8825782] |
| Author | Doig, P., Kinsella, N., Guerry, P. and Trust, T.J |
| Research Group | Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada. |
| Corresponding Author | Trust, T.J. |
| Contact | Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada. |
| Reference | Logan, S.M., Trust, T.J. and Guerry, P. (1989) Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. J Bacteriol, 171, 3031-3038. [PubMed: 2722741] |
| Author | Logan, S.M., Trust, T.J. and Guerry, P |
| Corresponding Author | Guerry, P |
| Reference | Guerry, P., Doig, P., Alm, R.A., Burr, D.H., Kinsella, N. and Trust, T.J. (1996) Identification and characterization of genes required for post-translational modification of Campylobacter coli VC167 flagellin. Mol Microbiol, 19, 369-378. [PubMed: 8825781] |
| Author | Guerry, P., Doig, P., Alm, R.A., Burr, D.H., Kinsella, N. and Trust, T.J |
| Research Group | Enteric Diseases Program, Naval Medical Research Institute, Bethesda, Maryland 20889, USA. |
| Corresponding Author | Trust, T.J. |
| Contact | Enteric Diseases Program, Naval Medical Research Institute, Bethesda, Maryland 20889, USA. |
