ProGP518 (Slg1 (S-layer glycoprotein))
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| ProGP ID | ProGP518 (Slg1 (S-layer glycoprotein)) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Haloarcula hispanica strain ATCC 33960 |
| Domain | Archaea |
| Classification | Phylum : Euryarchaeota Class : Halobacteria Orders : Haloferacales Family : Haloarculaceae Genus : Haloarcula Species : hispanica Strain : ATCC 33960 |
| Taxonomic ID (NCBI) | 634497 |
| Genome Information | |
| GenBank | CP002921 |
| EMBL | CP002921 |
| Organism Additional Information | H. hispanica, a halophilic archaeon, is extraordinary among members of the Halobacteriaceae in that it displays particularly low restriction activity and is therefore important as one of the most tractable haloarchaea for archaeal genetic research. The membrane contains a high content of carotenoid pigments (bacterioruberin) which give red pigmentation to the cells. It needs at least 3.4 M NaCl (higher than that needed by H. volcanii) for good growth. |
| Gene Information | |
| Gene Name | Hah_1991 |
| NCBI Gene ID | 11050867 |
| GenBank Gene Sequence | NC_015948.1 |
| Protein Information | |
| Protein Name | Slg1 (S-layer glycoprotein) |
| UniProtKB/SwissProt ID | G0HV85 |
| NCBI RefSeq | WP_014040752.1 |
| EMBL-CDS | AEM57587.1 |
| UniProtKB Sequence | >tr|G0HV85|G0HV85_HALHT Uncharacterized protein OS=Haloarcula hispanica (strain ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755) GN=HAH_1991 PE=4 SV=1 MTDTNEKIRSLFLTALMVFSVFAGTIAFSGGAAAAANVSVQQAAEYDSGTVELALNGSTG STVNTGDIDIYVDGNKNPSNYGVSSVDTTDDGTTGRLQFSLDQDVQPNRNLTVKVSGLTG GDNTVVAEDIDVTSQTIDADDDSGDTNAFRGEVLAIRADGDTDNDDATSSTKIVVEDSNG AVVTQDTYTANSKVYTYETENLDTGEEYEVSVAGDADENITISNLDLNVNIDDDVGDGAN IDDKDTLAVNVSTTRGGEPANATLFNEDDDKVATQVESLKGNENVVFDFGNQSADDSPYY VKVTDNQTGVSAESDQINVSESDDGEASFESSTVQDNIGDVVNITVQVDNTEDAVINIGD ENDDNYYIQGQLEDDNGDGEVTVQFNSYTAGNYSDSTVLSVPGDDDIDNIKEGGDYTRGS LSGDTLEAGSYSMNVTAGTSPDVTSPDTVGTLRLNENSVENIQTWAAPSDADIDDDDVDI YDRIGENLTQSDDVAAEDVVVHEIQASGIEGALEYEQEDGSASDVTEAFIAATDTTPYRI NDSDATTSGLRLYVNRTDVGANADANPIDFSNSSDAVTVVDDPDNNTYFVAVDTSDVVFE NGKTIVKEEDTRLNATFSVREGPLTDDRNSDSAIYTTSERDATLDLDDSGVVTVSAAAGQ EVTGETNVAPGSELEVEMESESDANPFVIRPEVDVATDGTYTATADFQDYSAGTNFTVQT LDVDGDSDFSDEEDGRIVEADTATVSISEQESDGSEVVVDSAQLSEGGFIAIHAGNASGD VVGNSEYLGAGSHEDITVTLDEPMDEDFTAVAMPHQDTNGNEAYDFPGDDDPYTQNGSAV TDSANVTIVEEEQTEAPDTETETEAPDTETEEETDAPATDEPATDESETTAAEGPGFTAA IALIALVAAALLAVRRDN |
| Sequence length | 918 AA |
| Function | The number and similarity of the S-layer glycoproteins may be used as a potential taxonomic marker for halophilic archaea. In addition to this, S-layer glycoproteins might be helpful as reporter molecules for studying the origin and evolution of archaea. |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) and O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N306 |
| Glycosite(s) Annotated Protein Sequence | >tr|G0HV85|G0HV85_HALHT Uncharacterized protein OS=Haloarcula hispanica (strain ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755) GN=HAH_1991 PE=4 SV=1 MTDTNEKIRSLFLTALMVFSVFAGTIAFSGGAAAAANVSVQQAAEYDSGTVELALNGSTG STVNTGDIDIYVDGNKNPSNYGVSSVDTTDDGTTGRLQFSLDQDVQPNRNLTVKVSGLTG GDNTVVAEDIDVTSQTIDADDDSGDTNAFRGEVLAIRADGDTDNDDATSSTKIVVEDSNG AVVTQDTYTANSKVYTYETENLDTGEEYEVSVAGDADENITISNLDLNVNIDDDVGDGAN IDDKDTLAVNVSTTRGGEPANATLFNEDDDKVATQVESLKGNENVVFDFGNQSADDSPYY VKVTDN*(305)QTGVSAESDQINVSESDDGEASFESSTVQDNIGDVVNITVQVDNTEDAVINIGD ENDDNYYIQGQLEDDNGDGEVTVQFNSYTAGNYSDSTVLSVPGDDDIDNIKEGGDYTRGS LSGDTLEAGSYSMNVTAGTSPDVTSPDTVGTLRLNENSVENIQTWAAPSDADIDDDDVDI YDRIGENLTQSDDVAAEDVVVHEIQASGIEGALEYEQEDGSASDVTEAFIAATDTTPYRI NDSDATTSGLRLYVNRTDVGANADANPIDFSNSSDAVTVVDDPDNNTYFVAVDTSDVVFE NGKTIVKEEDTRLNATFSVREGPLTDDRNSDSAIYTTSERDATLDLDDSGVVTVSAAAGQ EVTGETNVAPGSELEVEMESESDANPFVIRPEVDVATDGTYTATADFQDYSAGTNFTVQT LDVDGDSDFSDEEDGRIVEADTATVSISEQESDGSEVVVDSAQLSEGGFIAIHAGNASGD VVGNSEYLGAGSHEDITVTLDEPMDEDFTAVAMPHQDTNGNEAYDFPGDDDPYTQNGSAV TDSANVTIVEEEQTEAPDTETETEAPDTETEEETDAPATDEPATDESETTAAEGPGFTAA IALIALVAAALLAVRRDN |
| Sequence Around Glycosites (21 AA) | DSPYYVKVTDNQTGVSAESDQ |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff staining, ConA binding and TFMS deglycosylation |
| Technique(s) used for Glycosylated Residue(s) Detection | MALDI-TOF MS and MS/MS |
| Glycan Information | |
| Glycan Annotation | N-glycan was identified as a branched trisaccharide containing a 225 Da residue which corresponds to a 2-amino-6-sulfo-2, 6-dideoxy-quinovose. Glcβ-1,4-Gal disaccharide was identified as the O-glycan. Acidic trisaccharides linked to N306. |
| Technique(s) used for Glycan Identification | ESI-MS, MALDI-TOF, MS/MS after aminobenzamide (2-AB) labeling and NMR (DQF-COSY, HSQC, TOCSY and HMBC) |
| Protein Glycosylation linked (PGL) gene(s) | |
| Additional Comment | This is the first time that a naturally occurring form of sulfoquinovosamine has been identified. |
| Literature | |
| Year of Identification | 2015 |
| Year of Identification Month Wise | 2015.11.1 |
| Year of Validation | 2015 |
| Reference | Lu, H., Lü, Y., Ren, J., Wang, Z., Wang, Q., Luo, Y., Han, J., Xiang, H., Du, Y. and Jin, C., 2015. Identification of the S-layer glycoproteins and their covalently linked glycans in the halophilic archaeon Haloarcula hispanica. Glycobiology, 25(11), pp.1150-1162. |
| Corresponding Author | Cheng Jin |
| Contact | State Key Laboratory of Mycology |