ProGP522 (Putative membrane fusion protein)

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ProGP ID ProGP522 (Putative membrane fusion protein)
Validation Status Characterized
Organism Information
Organism NameRalstonia solanacearum GMI1000
Domain Bacteria
Classification Phylum : Proteobacteria
Class : Betaproteobacteria
Orders : Burkholderiales
Family : Burkholderiaceae
Genus : Ralstonia
Species : solanacearum
Strain : GMI1000
Taxonomic ID (NCBI) 267608
Genome Information
GenBank NC_003295.1
EMBL AL646053.1
Gene Information
Gene NameRSp1197
NCBI Gene ID 1223509
GenBank Gene Sequence NC_003296.1
Protein Information
Protein NamePutative membrane fusion protein
UniProtKB/SwissProt ID Q8XQM5
NCBI RefSeq WP_011004483.1
EMBL-CDSCAD18348.1
UniProtKB Sequence >tr|Q8XQM5|Q8XQM5_RALSO Putative membrane fusion protein OS=Ralstonia solanacearum (strain GMI1000) GN=RSp1197 PE=3 SV=1 MSDAMDPKPGTPYAGHDTVSPTLLPPPRAGKPRRWILWVVLAVLVLIGYAIFHAVHRSTA AGKGGPGGRGAAMTSGKPMPVMVATSGKGDINVVLTALGNVTPVNNVTVKTRVDGQLVRL AFTEGQTVKAGDLLAEIDPRAYQAQLAQAEGQLARDTALLQNARLDLQRYQTLMAQDSIA KQQVDTQASLVRQYEGTVKLDQGNADNARVQLSYTRITAPVSGRVGLRQVDPGNIVHTSD TTGIVVITQIDPITVIYTLPEDTLPKVMPRLQAGDKLPVEAWDRGQTNLLAHGTLMTVDN TIDNTTGTVKLRAQFPNQNAALFPNQFVNVRMRVDTLHDQVILPGAAIQRGTQGTFVYVV GADGNVALRTVTLGVTEGDRVSIARGLNPGERVVIDGADKLRDGSPVEVIQPGASPASAP AAAHDGQAAQAQGGHRRHGGASAPAAAQP
Sequence length 449 AA
Subcellular LocationMembrane
Function RND transporter MFP subunit
Glycosylation Status
Glycosylation Type O- (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS418 and S442
Glycosite(s) Annotated Protein Sequence >tr|Q8XQM5|Q8XQM5_RALSO Putative membrane fusion protein OS=Ralstonia solanacearum (strain GMI1000) GN=RSp1197 PE=3 SV=1 MSDAMDPKPGTPYAGHDTVSPTLLPPPRAGKPRRWILWVVLAVLVLIGYAIFHAVHRSTA AGKGGPGGRGAAMTSGKPMPVMVATSGKGDINVVLTALGNVTPVNNVTVKTRVDGQLVRL AFTEGQTVKAGDLLAEIDPRAYQAQLAQAEGQLARDTALLQNARLDLQRYQTLMAQDSIA KQQVDTQASLVRQYEGTVKLDQGNADNARVQLSYTRITAPVSGRVGLRQVDPGNIVHTSD TTGIVVITQIDPITVIYTLPEDTLPKVMPRLQAGDKLPVEAWDRGQTNLLAHGTLMTVDN TIDNTTGTVKLRAQFPNQNAALFPNQFVNVRMRVDTLHDQVILPGAAIQRGTQGTFVYVV GADGNVALRTVTLGVTEGDRVSIARGLNPGERVVIDGADKLRDGSPVEVIQPGASPAS*(418)AP AAAHDGQAAQAQGGHRRHGGAS*(442)APAAAQP
Sequence Around Glycosites (21 AA) EVIQPGASPASAPAAAHDGQA
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Technique(s) used for Glycosylation DetectionZIC-HILIC
Technique(s) used for Glycosylated Residue(s) Detection LC-MS, CID MS/MS and HCD MS/MS
Glycan Information
Glycan Annotation Pentasaccharide (HexNAc-(Pen)-dHex3)
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamePglLRs
Literature
Year of Identification2016
Year of Identification Month Wise2016.3.26
Year of Validation 2016
ReferenceElhenawy, W., Scott, N.E., Tondo, M.L., Orellano, E.G., Foster, L.J. and Feldman, M.F., 2016. Protein O-linked glycosylation in the plant pathogen Ralstonia solanacearum. Glycobiology, 26(3), pp.301-311.
Corresponding Author Mario F Feldman
ContactDepartment of Biological Sciences, University of Alberta, Edmonton, AB, Canada Department of Molecular Microbiology, Washington University School of Medicine St. Louis, St. Louis, MO, USA