ProGP546 (Pilin A2)
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| ProGP ID | ProGP546 (Pilin A2) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Haloferax volcanii H53 |
| Domain | Archaea |
| Classification | Phylum : Euryarchaeota Class : Halobacteria Orders : Haloferacales Family : Haloferacaceae Genus : Haloferax Species : volcanii Strain : H53 |
| Taxonomic ID (NCBI) | 309800 |
| Genome Information | |
| GenBank | CP001956.1 |
| EMBL | CP001956.1 |
| Gene Information | |
| Gene Name | pilA2 (HVO_2062) |
| NCBI Gene ID | 8923886 |
| GenBank Gene Sequence | NC_013967.1 |
| Protein Information | |
| Protein Name | Pilin A2 |
| UniProtKB/SwissProt ID | D4GU75 |
| NCBI RefSeq | WP_004041947.1 |
| EMBL-CDS | ADE03103.1 |
| UniProtKB Sequence | >tr|D4GU75|D4GU75_HALVD Pilin PilA OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=pilA2 PE=4 SV=1 MQLTTLFNDDSAVSPVIGVILMVAITVILAAVIGTFVLGLGDQVSETSPQASFDFDYTNT SGNLTITHESGTSIDADSVSISGPVGDDGKTWADIDGSATEITAGSSITVTANGSSFDSG ETVRVIWTSDSGSSSSTLQSWTYNG |
| Sequence length | 145 AA |
| Function | Structural subunit of type IV pili involved in surface adhesion |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N59, N63 and N113 |
| Glycosite(s) Annotated Protein Sequence | >tr|D4GU75|D4GU75_HALVD Pilin PilA OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=pilA2 PE=4 SV=1 MQLTTLFNDDSAVSPVIGVILMVAITVILAAVIGTFVLGLGDQVSETSPQASFDFDYTN*(59)T SGN*(63)LTITHESGTSIDADSVSISGPVGDDGKTWADIDGSATEITAGSSITVTAN*(113)GSSFDSG ETVRVIWTSDSGSSSSTLQSWTYNG |
| Sequence Around Glycosites (21 AA) | PQASFDFDYTNTSGNLTITHE FDFDYTNTSGNLTITHESGTS TAGSSITVTANGSSFDSGETV |
| Technique(s) used for Glycosylation Detection | Aberrant migration on lithium dodecyl sulfate (LDS)-PAGE as detected through western blotting |
| Technique(s) used for Glycosylated Residue(s) Detection | MS using HCD fragmentation |
| Protein Glycosylation- Implication | N-glycosylation may regulate the transition from planktonic cell to biofilm formation under stress conditions. |
| Glycan Information | |
| Glycan Annotation | One tetra- and one pentasaccharide on its two glycosites. The pentasaccharide is composed of a hexose, two hexuronic acids, a methylated hexuronic acid, and a mannose. |
| Technique(s) used for Glycan Identification | MS using IS (in source)-CID and HCD fragmentation |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | AglB |
| Additional Comment | Pilin expression in pilA[1-6] aglB stimulates microcolony formation. |
| Literature | |
| Year of Identification | 2016 |
| Year of Identification Month Wise | 2016.05 |
| Year of Validation | 2016 |
| Reference | Esquivel, R.N., Schulze, S., Xu, R., Hippler, M. and Pohlschroder, M., 2016. Identification of Haloferax volcanii pilin N-glycans with diverse roles in pilus biosynthesis, adhesion, and microcolony formation. Journal of Biological Chemistry, 291(20), pp.10602-10614. |
| Corresponding Author | Mechthild Pohlschroder |
| Contact | From the Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 |