| Technique(s) used for Glycosylation Detection | Deglycosylation (trifluoromethanesulfonic acid), aberrant migration on SDS-PAGE. |
| Technique(s) used for Glycosylated Residue(s) Detection | Protease digestion and glycopeptide separation by molecular sieve chromatography and reversed-phase HPLC. Deglycosylated and undeglycosylated peptides were run on 2D PAGE and subjected to N-terminal sequencing post blotting. |
| Protein Glycosylation- Implication | An N terminal SLH sequence of A. kivui S layer glycoprotein is suggested to fold independently and present itself in a modular way that may serve as an anchor to the peptidoglycan. Glycosylated residues are surface exposed and implicated in protease K resistance. |
| Glycan Information |
| Glycan Annotation | Carbohydrate content 8% corresponding to 40 to 50 sugar molecules per protein monomer. Acidic, repetitive heterogenous glycans of varying length containing N-acetylglucosamine, 1,6 linked glucose, 1,4 linked galactosamine and/or unidentified sugar-related component. |
| Technique(s) used for Glycan Identification | Hydrazinolysis followed by amino-acid analysis as well as two-dimensional NMR spectroscopy |
| Literature |
| Year of Identification | 1989 |
| Year of Identification Month Wise | 1989.11.01 |
| Year of Validation | 1992 |
| Reference | Lupas, A., Engelhardt, H., Peters, J., Santarius, U., Volker, S. and Baumeister, W., 1994. Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis. Journal of bacteriology, 176(5), pp.1224-1233. |
| Corresponding Author | W Baumeister |
| Contact | Max-Planck-Institut für Biochemie, Martinsried, Germany |
| Reference | PETERS, J., RUDOLF, S., OscHKiNAT, H., MENGELE, R., Sumper, M., KELLERMANN, J., LOTTSPEICH, F. and BAUMEISTER, W., 1992. Evidence for tyrosine-linked glycosaminoglycan in a bacterial surface protein. |
| Corresponding Author | Jurgen Peters |
| Contact | Max-Planck-Institut für Biochemie, Martinsried bei München. |
| Reference | Peters, J., Peters, M., Lottspeich, F. and Baumeister, W., 1989. S-layer protein gene of Acetogenium kivui: cloning and expression in Escherichia coli and determination of the nucleotide sequence. Journal of bacteriology, 171(11), pp.6307-6315. |
| Corresponding Author | Jurgen Peters |
| Contact | Max-Planck-Institut für Biochemie, Martinsried bei München. |
| Reference | Lupas, A., Engelhardt, H., Peters, J., Santarius, U., Volker, S. and Baumeister, W. (1994) Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis. J Bacteriol, 176, 1224-1233. [PubMed: 8113161] |
| Author | Lupas, A., Engelhardt, H., Peters, J., Santarius, U., Volker, S. and Baumeister, W. |
| Research Group | Max Planck Institute for Biochemistry, Martinsried, Germany |
| Corresponding Author | Baumeister, W. |
| Contact | Max Planck Institute for Biochemistry, Martinsried, Germany |
| Reference | Peters, J., Rudolf, S., Oschkinat, H., Mengele, R., Sumper, M., Kellermann, J., Lottspeich, F. and Baumeister, W. (1992) Evidence for tyrosine-linked glycosaminoglycan in a bacterial surface protein. Biol Chem Hoppe Seyler, 373, 171-176. [PubMed: 1596358] |
| Author | Peters, J., Rudolf, S., Oschkinat, H., Mengele, R., Sumper, M., Kellermann, J., Lottspeich, F. and Baumeister, W. |
| Research Group | Max Planck Institute for Biochemistry, Martinsried, Federal Republic of Germany. |
| Corresponding Author | Baumeister, W. |
| Contact | Max Planck Institute for Biochemistry, Martinsried, Federal Republic of Germany. |
| Reference | Peters, J., Peters, M., Lottspeich, F., Baumeister, W.(1989) S-layer protein gene of Acetogenium kivui: cloning and expression in Escherichia coli and determination of the nucleotide sequence. J Bacteriol, 171, 6307-6315. [PubMed: 2681162] |
| Author | Peters, J., Peters, M., Lottspeich, F. Baumeister, W |
| Research Group | Max Planck Institute for Biochemistry, Martinsried, Federal Republic of Germany. |
| Corresponding Author | F. Baumeister, W |
| Contact | Max Planck Institute for Biochemistry, Martinsried, Federal Republic of Germany. |
