ProGP563 (Pullulanase)
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| ProGP ID | ProGP563 (Pullulanase) |
| Validation Status | Uncharacterized |
| Organism Information | |
| Organism Name | Pyrobaculum calidifontis JCM 11548 |
| Domain | Archaea |
| Classification | Family:Thermoproteaceae Order: Thermoproteales Class:Thermoprotei Division or phylum: "Crenarchaeota" |
| Taxonomic ID (NCBI) | 410359 |
| Genome Information | |
| GenBank | CP000561.1 |
| EMBL | CP000561 |
| Organism Additional Information | Pyrobaculum calidifontis is a hyperthermophilic archaeon that belongs to the phylum Crenarchaeota. In contrast to the phylum Euryarchaeota, only the N-glycan structure of the genus Sulfolobus is known in Crenarchaeota. |
| Gene Information | |
| Gene Name | Pcal_1616 |
| Protein Information | |
| Protein Name | Pullulanase |
| UniProtKB/SwissProt ID | A3MWL6 |
| NCBI RefSeq | ABO09033.1. |
| EMBL-CDS | ABO09033.1. |
| UniProtKB Sequence | >tr|A3MWL6|A3MWL6_PYRCJ Pullulanase / alpha-amylase OS=Pyrobaculum calidifontis (strain JCM 11548 / VA1) GN=Pcal_1616 PE=3 SV=1 MKILALLLSALVLAQTVNVVVILHNHQPWYIDLERGELVLPWVRMHAVGNYLKVPLLINQ TGVSVAFTLSGSLIEQLNWYANGTYLDARFKISEKLARGEALTAEEKYSMLKVPGGFFDI NWQNILYKNPRYTALLGIRNDAFNKCPPGDVTCVVSKFSDQDFVDLATLFNLMWIDPYIA RQRPDIWALRNKTSFTRDDLAKVLQFHIELIKEVLPLYKRLAEQGRIELVPVPYSHPLMP LLADMGAVDDLRLHIQLSNGLFRRYLGASPLGVWPPEQAVNDEVLRLFAEEGYLWTVTDE DVLKMTMPGKSHFQLYYADYGGRRIYVFFRDKTLSDNIGFRYSSMSPQAALADFVNYLRR VPRGDCNVVVIALDGENPWENYPNFGDDFLLQFFGGLAQLEKNGTIKLWKPTDFVKACGD KAEPLPQREFQYYNLGVDISFYNSIRELPTRTVLGKIAEGSWSSGGSLAVWIGDPDENAW WMWLKKAREDVGAAKSWDVLFPLLVAEASDWPFWYGGDMGSPQTFDPVAKAALRAYYQRA GLEPPAYLYTTAYPAGIPREDKVAGQGHGSVKAQDATIYVNTTHVWVSGGRCGVVYISNP NVPRSPYVPRGAVFGLRGERLDIYADMALDTCNGTVYLADGGKFVAVGNNALLSLIGAKP GGKLYVEFNGFVYVLGIPETTTSARLVMSAEDPPGDDFGPGSYNYPKNPAFRPGVFDLLG MEVYDLGDKLRFVFRVRELGGNPWGGPAGFSLQFFHVYINRGRGTRNDTLGLRVALCRDA AWDAALLIGPGWSGGNRIVFADGSFIDDAISIRAGPNNTVVADVPKKYIGDFDPKWRLTV FLTSWDGYGPDNIRNFGVMADEWTVGGADPAAVLAGVAPRVFDLLAPAAEEQKKALSTYK VSRLPNGTYVGTPATVCTYVSPSRAETQTTTVVQTVVQYVTQQTTETVTRETTYTTTYIS TTTTTLVEKVVDWGIAAIPVALMALITVAAIALALALAKRRPRQSI |
| Sequence length | 1006 AA |
| Subcellular Location | Integral component of membrane |
| Function | Pullulanase / alpha-amylase |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Technique(s) used for Glycosylation Detection | ConA(Concanavalin A) lectin affinity chromatography , and detected by periodic acid-Schiff (PAS) staining and Coomassie Brilliant Blue (CBB) staining on sodium dodecyl sulphate?polyacrylamide gel electrophoresis (SDS?PAGE) gels |
| Technique(s) used for Glycosylated Residue(s) Detection | Normal-phase-HPLC-MS/MS analysis |
| Glycan Information | |
| Glycan Annotation | Pentasaccharide (α-Man-(1-3)-(α-Man-(1-6)-)α-Man-(1-4)-β-GlcANAc3NAc-(1-4)-β-GlcNAc3NAc) |
| GlyTouCan | G06484YZ G62526ZK |
| Literature | |
| Year of Identification | 2017 |
| Year of Validation | 2017 |
| Reference | Fujinami, D., Taguchi, Y. and Kohda, D., 2017. Asn-linked oligosaccharide chain of a crenarchaeon, Pyrobaculum calidifontis, is reminiscent of the eukaryotic high-mannose-type glycan. Glycobiology, 27(8), pp.701-712. |
| Corresponding Author | Daisuke Kohda |
| Contact | 1. Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. 2. Research Center for Advanced Immunology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan._x000D_ 3. Research Center for Live-Protein Dynamics, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. |
| Reference | Fujinami D, Taguchi Y, Kohda D.(2017) Asn-linked oligosaccharide chain of a crenarchaeon, Pyrobaculum calidifontis, is reminiscent of the eukaryotic high-mannose-type glycan. Glycobiology, 16. [PubMed: 28510654] |
| Author | Fujinami D, Taguchi Y, Kohda D |
| Research Group | 1 Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. 2 Research Center for Advanced Immunology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. 3 Research Center for Live-Protein Dynamics, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. |
| Corresponding Author | Kohda D |
| Contact | 1 Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. 2 Research Center for Advanced Immunology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. 3 Research Center for Live-Protein Dynamics, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. |