| Technique(s) used for Glycosylation Detection | Concanavalin A (ConA)-binding, DIG glycan detection (labeling with digoxigenin-succinyl-epsilon-amidocaproic acid hydrazide and anti-DIG antibody after periodate oxidation) |
| Technique(s) used for Glycosylated Residue(s) Detection | Q-TOF nanoelectrospray MS-MS (hybrid quadrupole orthogonal acceleration time of flight), deletion analysis and site-directed mutagenesis |
| Protein Glycosylation- Implication | Glycosylation may function either as a signal for cleavage or as a means of preventing amino-terminal degradation of the protein following cleavage from its acylated anchor. This is because the 23-kDa form of MPB83 is generated by proteolytic cleavage immediately before Thr48 from the mature acylated 25-kDa form. |
| Glycan Information |
| Glycan Annotation | Linkage: Man-Thr.
3 mannose units present with Man(1?3)Man linkage. The dominant glycoform identified for MPB83 is Thr48 substituted with a single mannose residue and Thr49 substituted with a Man(1?3)Man linkage. However, evidence for a heterogeneous array of glycoforms from Thr48(Man3) Thr49(Man0) through to Thr48(Man0)Thr49(Man3) has also been observed. |
| BCSDB ID | 3436 |
| Technique(s) used for Glycan Identification | Dionex ion chromatography of sugars after their release by acid hydrolysis, FAB-MS (fast atom bombardment-mass spectrometry) of permethylated O-glycans after their release by reductive elimination, and GC-MS linkage analysis of the partially methylated alditol acetates, derived from the permethylated products of reductive elimination. |
| Literature |
| Year of Identification | 1991 |
| Year of Identification Month Wise | 1991.03 |
| Year of Validation | 2003 |
| Reference | Michell, S.L., Whelan, A.O., Wheeler, P.R., Panico, M., Easton, R.L., Etienne, A.T., Haslam, S.M., Dell, A., Morris, H.R., Reason, A.J. et al. (2003) The MPB83 antigen from Mycobacterium bovis contains O-linked mannose and (1-->3)-mannobiose moieties. J Biol Chem, 278, 16423-16432. [PubMed: 12517764] |
| Author | Michell, S.L., Whelan, A.O., Wheeler, P.R., Panico, M., Easton, R.L., Etienne, A.T., Haslam, S.M., Dell, A., Morris, H.R., Reason, A.J. |
| Research Group | TB Research Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK |
| Corresponding Author | Michell, S.L. |
| Contact | TB Research Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK |
| Reference | Fifis, T., Costopoulos, C., Radford, A.J., Bacic, A. and Wood, P.R. (1991) Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate. Infect Immun, 59, 800-807. [PubMed: 1900061] |
| Author | Fifis, T., Costopoulos, C., Radford, A.J., Bacic, A. Wood, P.R. |
| Research Group | Commonwealth Scientific and Industrial Research Organisation, Division of Animal Health, Parkville, Victoria Australia. |
| Corresponding Author | Wood, P.R. |
| Contact | Commonwealth Scientific and Industrial Research Organisation, Division of Animal Health, Parkville, Victoria Australia. |
