Dionex ion chromatography of sugars after their release by acid hydrolysis, FAB-MS (fast atom bombardment-mass spectrometry) of permethylated O-glycans after their release by reductive elimination, and GC-MS linkage analysis of the partially methylated alditol acetates, derived from the permethylated products of reductive elimination.
Protein Glycosylation linked (PGL) gene(s)
Additional Comment
23-kDa is the predominant form of the antigen in the culture supernatant. 25/23-kDa antigen was misidentified as glycosylated form of MPB70. It is actually MPB83 antigen.
TB Research Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK. slmichell@dstl.gov.uk