ProGT1.2 (WbpL)

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ProGT ID ProGT1.2 (WbpL)
ProGT Pathway
Organism Information
Organism NamePseudomonas aeruginosa 1244
Domain Bacteria
Classification Phylum : Proteobacteria
Class : Gammaproteobacteria
Orders : Pseudomonadales
Family : Pseudomanadaceae
Genus : Pseudomonas
Species : aeruginosa
Taxonomic ID (NCBI)287
Genome Information
Gene Information
Gene NamewbpL 
NCBI Reference SequenceAAM21928.1
Protein information
Protein NameWbpL 
UniProtKB/ SwissProt IDQ8KHQ7
UniProtKB Sequence>tr|Q8KHQ7|Q8KHQ7_PSEAI Serotype 07 putative O-antigen biosynthesis gene cluster, partial sequence OS=Pseudomonas aeruginosa GN=wbpL PE=4 SV=1 MNQWIIEMFFFLILAVISVSCAGTWLLRRYALARSIIDVPNARSSHSVPTPRGGGVAIVI SYLALLPVLVALDVVTAPLAIALGGGAAIALLGFLDDHGHIAARWRLLGHFSAAGWGLFW LGGFPALKFIGIHIEAGWVLNSLAALYVVWMLNLYNFMDGIDGIASVEAITACTLASLIF WGVGQQQLLIAPLFLAAAVAGFLIWNFPPAKIFMGDAGSGFLGITLAVLSMHAAWVSMDF FWSWLILLGVFVTDATVTLIRRLMRGEKVYEAHRSHAYQFAARRFGQHLPVTLFVGAINI VWLAPIAWSVARGLLPGELGVVIAYLPLVFLAVRYRAGQMEHIIAR
EMBL CDSAAM21928.1.
Sequence length346 AA
Subcellular LocationMembrane (Integral component of membrane)
Glycosylation Information
Sugar Donor SpecificityUDP-FucNAc 
Acceptor Substrate SpecificityUndPP
Experimental ValidationIn vivo
ProductUndPP-FucNAc
Donor SpecificityUDP-FucNAc
Function in Glycosylation pathway1) WbpL, a bifunctional glycosyltransferase, is required for the initiation of both LPS and O antigen synthesis.
2) WbpL involved in the initiation of the assembly of individual O?antigen units by the transfer of FucNAc from UDP?FucNAc to undecaprenol phosphate.
Additional Information1) Expression of O-antigen biosynthesis cluster of Escherichia coli O157:H7 in strain P. aeruginosa 1244 resulted in the production of pilin that contained both the endogenous Pseudomonas as well as the Escherichia O157 O-antigens. 
Litrature
Year Of Validation2002 
Reference DiGiandomenico, A., Matewish, M.J., Bisaillon, A., Stehle, J.R., Lam, J.S. and Castric, P., 2002. Glycosylation of Pseudomonas aeruginosa 1244 pilin: glycan substrate specificity. Molecular microbiology, 46(2), pp.519-530.

Corresponding AuthorDepartment of Biological Sciences, Duquesne University, Pittsburg, PA 15282, USA.