| ProGT ID | ProGT75.5 (GlyE) |
| Organism Information | |
| Organism Name | Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Streptococcaceae Order: Lactobacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 170187 |
| Genome Information | |
| Gene Bank | AE005672 |
| EMBL | AE005672 |
| Gene Information | |
| Gene Name | glyE (SP_1766)Â |
| Protein information | |
| Protein Name | GlyEÂ |
| UniProtKB/ SwissProt ID | A0A0H2URJ6 |
| NCBI Ref Seq | WP_001808456.1 |
| UniProtKB Sequence | >tr|A0A0H2URJ6|A0A0H2URJ6_STRPN Glycosyl transferase, family 8 OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_1766 PE=4 SV=1 MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIPQEWFSQIRIYLQEMGG DLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKVLYLDSDLIVTGDLTDLFE LDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQKLIDLTEKEHENVEEGDQSI LNMLFKDQYSSLEDQYNFQIGYDYGAATFKHQFIFDIPLEPLPLILHYISQDKPWNQFSV GRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQLP EVHFHIVAYTNMANELLALTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYE FVLKYKKPMIAFDNTCSENLSEISYEGIYPSSIPKKMVAAIRSYMR |
| EMBL CDS | AAK75841 |
| Sequence length | 406 AA |
| PDB ID (Structural Information) | 5GVV, 5GVW |
| Glycosylation Information | |
| Sugar Donor Specificity | UDP-Gal |
| Acceptor Substrate Specificity | SRR1-GlcNAc-Glc-Glc |
| Experimental Validation | In vivo and In vitro |
| Product | SRR1-GlcNAc-Glc-Glc-Gal |
| Donor Specificity | UDP-Gal |
| Function in Glycosylation pathway | 1) GlyE is galactosyltransferase uses UDP-Gal as the donor, SRR1-GlcNAc-Glc-Glc is further modified to produce SRR1-GlcNAc-Glc-Glc-Gal. |
| Additional Information | 1) GlyE shows a higher hydrolytic activity toward UDP-Glc compared with UDP-Gal. Â |
| Litrature | |
| Year Of Validation | 2017Â |
| Reference | Jiang, Y. L., Jin, H., Yang, H. B., Zhao, R. L., Wang, S., Chen, Y., & Zhou, C. Z. (2017). Defining the enzymatic pathway for polymorphic O-glycosylation of the pneumococcal serine-rich repeat protein PsrP. The Journal of Biological Chemistry, 292, 6213-6224. |
| Authors | Jiang, Y. L., Jin, H., Yang, H. B., Zhao, R. L., Wang, S., Chen, Y., & Zhou, C. Z. |
| Research groups | 1 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China.
2 Key Laboratory of Structural Biology, Chinese Academy of Science, Hefei, Anhui 230027, China.
3 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China.
4 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China |
| Corresponding Author | Zhou, C. Z. |
| Contacts | 1 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China. |