| ProGT ID | ProGTNC9 (WbpE) |
| Organism Information | |
| Organism Name | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PR |
| Domain | Bacteria |
| Phylum | Proteobacteria |
| Classification | Family: Pseudomonadaceae Order: Pseudomonadales Class: Gammaproteobacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 208964 |
| Genome Information | |
| Gene Bank | AE004091 |
| EMBL | AE004091 |
| Gene Information | |
| Gene Name | wbpE |
| NCBI Gene ID | 882653 |
| Protein information | |
| Protein Name | WbpE |
| UniProtKB/ SwissProt ID | Q9HZ76 |
| NCBI Ref Seq | WP_003113425.1 |
| UniProtKB Sequence | >sp|Q9HZ76|WBPE_PSEAE UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=wbpE PE=1 SV=1 MIEFIDLKNQQARIKDKIDAGIQRVLRHGQYILGPEVTELEDRLADFVGAKYCISCANGT DALQIVQMALGVGPGDEVITPGFTYVATAETVALLGAKPVYVDIDPRTYNLDPQLLEAAI TPRTKAIIPVSLYGQCADFDAINAIASKYGIPVIEDAAQSFGASYKGKRSCNLSTVACTS FFPSKPLGCYGDGGAIFTNDDELATAIRQIARHGQDRRYHHIRVGVNSRLDTLQAAILLP KLEIFEEEIALRQKVAAEYDLSLKQVGIGTPFIEVNNISVYAQYTVRMDNRESVQASLKA AGVPTAVHYPIPLNKQPAVADEKAKLPVGDKAATQVMSLPMHPYLDTASIKIICAALTN |
| EMBL CDS | AAG06543 |
| Sequence length | 359 AA |
| String | 208964.PA3155 |
| PDB ID (Structural Information) | 3NU7, 3NU8, 3NUB, 3NYS, 3NYT, 3NYU |
| Glycosylation Information | |
| EC Number (BRENDA) | 2.6.1.98 |
| Acceptor Substrate Specificity | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate |
| Product | UDP-2-acetamido-2-deoxy-D-ribo-hex-3-uluronate |
| Function in Glycosylation pathway | 1) Catalyzes the amination of UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronic acid (UDP-3-oxo-D-GlcNAcA) to UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid (UDP-GlcNAc3NA), using L-glutamate as the preferred amine donor. |
| Litrature | |
| Year Of Validation | 2008 |
| Reference | Westman EL, Preston A, Field RA, Lam JS. Biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharides of both Pseudomonas aeruginosa and Bordetella pertussis occurs via an identical scheme despite different gene clusters. Journal of bacteriology. 2008 Sep 15;190(18):6060-9. |
| Authors | Westman EL, Preston A, Field RA, Lam JS |
| Research groups | 1 University of Guelph, Department of Molecular and Cellular Biology, Guelph, Ontario, Canada. |
| Corresponding Author | Lam JS |
| Contacts | 1 University of Guelph, Department of Molecular and Cellular Biology, Guelph, Ontario, Canada. |
| Reference | Larkin A, Imperiali B. Biosynthesis of UDP-GlcNAc (3NAc) A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1. Biochemistry. 2009 May 22;48(23):5446-55. |
| Authors | Larkin A, Imperiali B |
| Research groups | 1 Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge,Massachusetts 02139, USA. |
| Corresponding Author | Imperiali B |
| Contacts | 1 Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge,Massachusetts 02139, USA. |
| Reference | Westman EL, McNally DJ, Charchoglyan A, Brewer D, Field RA, Lam JS. Characterization of WbpB, WbpE, and WbpD, and reconstitution of a pathway for the biosynthesis of UDP-2, 3-diacetamido-2, 3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa. Journal of Biological Chemistry. 2009 Mar 12. |
| Authors | Westman EL, McNally DJ, Charchoglyan A, Brewer D, Field RA, Lam JS |
| Research groups | 1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. |
| Corresponding Author | Lam JS |
| Contacts | 1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. |
| Reference | Thoden JB, Holden HM. Structural and functional studies of WlbA: A dehydrogenase involved in the biosynthesis of 2, 3-diacetamido-2, 3-dideoxy-D-mannuronic acid. Biochemistry. 2010 Aug 19;49(36):7939-48. |
| Authors | Thoden JB, Holden HM. |
| Research groups | 1 Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA. |
| Corresponding Author | Holden HM. |
| Contacts | 1 Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA. |