ProGT103 (EarP)
| ProGT ID | ProGT103 (EarP) |
| Organism Information | |
| Organism Name | Pseudomonas aeruginosa PAO1 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Pseudomonadales Family : Pseudomonadaceae Genus : Pseudomonas Species : aeruginosa Strain : PAO1 |
| Taxonomic ID (NCBI) | 208964 |
| Genome Information | |
| Gene Bank | AE004091 |
| EMBL | AE004091 |
| Gene Information | |
| Gene Name | PA2852 |
| NCBI Gene ID | 882634 |
| Protein information | |
| Protein Name | EarP |
| UniProtKB/ SwissProt ID | Q9HZZ1 |
| NCBI Ref Seq | AAG06240.1 |
| UniProtKB Sequence | >tr|Q9HZZ1|Q9HZZ1_PSEAE Uncharacterized protein OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) GN=PA2852 PE=4 SV=1 MASWDIFCSVVDNYGDIGVTWRLARQLAAEHGQAVRLWVDEPQAFARICPRADPVAHVQC LDGVEVRAWGRPWAPVAAADVVIEAFACELPEAHRQAMRERKRPSLWLNLEYLSAEEWIG SCHALPSLQACGLSKYFFFPGFREPSGGLLREAGLLERRRRFQASVSAQDEFLASLGVRR KVGERLISLFAYENPALPGWLEQLRDARQPSLLLVPEGRVLADVADWLRVATLAVGDVHV RDALRVQVLPFMAQDDYDRLLWCCDLNAVRGEDSFVRAQWAGRPLLWHIYRQEEETHLAK LEAFLELYCAGLPADLAENLRTFWLAWNAGGGLAGAWEGLERQLPEWRREAQRWADEQGM RPDLAARLVQFYADWL |
| EMBL CDS | AAG06240.1 |
| Sequence length | 376 AA |
| Function in Native Organism | 1) EarP and EF-P are essential for P. aeruginosa pathogenicity. |
| String | 208964.PA2852 |
| Potential Application | 1) Inhibitors targeting EarP type glycosyltransferase helps in the development of specific antibiotics against pathogens. |
| Additional Information | 1) EarP is an EF-P arginine rhamnosyltransferase which is essential for post-translational activation. 2) Virulence factors, such as rhamnolipids and pyocyanin are important to P. aeruginosa PAO1 for colonization and invasion during infection. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Arg) linked |
| CAZY Family | GT104 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | dTDP-l-Rhamnose |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Rha) |
| Method of Glycan Indentification | LC-MS/MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | EF-P |
| ProGPdb ID | ProGP495 |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Lassak, J., Keilhauer, E.C., Fürst, M., Wuichet, K., Gödeke, J., Starosta, A.L., Chen, J.M., Søgaard-Andersen, L., Rohr, J., Wilson, D.N. and Häussler, S., 2015. Arginine-rhamnosylation as new strategy to activate translation elongation factor P. Nature chemical biology, 11(4), pp.266-270. |
| Corresponding Author | 1 Center for Integrated Protein Science Munich, Ludwig-Maximilians-University Munich,, Munich, Germany. 2 Department of Biology I, Microbiology,Ludwig-Maximilians-University of Munich, Martinsried, Germany. |