ProGT109 (EarP)
| ProGT ID | ProGT109 (EarP) |
| Organism Information | |
| Organism Name | Neisseria meningitidis HT1125 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : BetaProteobacteria Orders : Neisseriales Family : Nesseriaceae Genus : Neisseria Species : meningitidis Strain : HT1125 |
| Taxonomic ID (NCBI) | 487 |
| Genome Information | |
| Gene Bank | AE002098.2 |
| EMBL | AE002098.2 |
| Gene Information | |
| Gene Name | earP |
| Protein information | |
| Protein Name | EarP |
| UniProtKB/ SwissProt ID | A0A0T7AQA7 |
| NCBI Ref Seq | YP_008920709.1 |
| UniProtKB Sequence | >tr|A0A0T7AQA7|A0A0T7AQA7_NEIME DUF2331 OS=Neisseria meningitidis GN=efp PE=4 SV=1 MNTPPFVCWIFCKVIDNFGDIGVSLRLARVLHRELGWQVHLWTDDVSALRALCPDLPDVP CVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLHIIRRHKPLWLNWEYLSAEE SNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRDAVRFDTEALRQRLMLPEKNAP EWLLFGYRSDVWAKWLEMWQQAGSPMTLLLAGAQIIDSLKQSGIIPQNALQNDGDVFQTA SVRLVKIPFVPQQDFDQLLHLADCAVIRGEDSFVRAQLAGKPFFWHIYPQDEHVHLDKLH AFWDKAHGFYTPETASAHRCLSDDLNGGEALSATQRLECWQILQQHQNGWRQGAGAWSRY LFGQPSASEKLAAFVSKHQKIR |
| EMBL CDS | BAU19337 |
| Sequence length | 382 AA |
| Function in Native Organism | 1) The N. meningitidis efp gene is essential for cell viability. |
| Potential Application | 1) EF-P and Arg32 are essential for the viability of the Neisseria meningitidis therefore, EarP should be a promising target for antibacterial drug development specifically against N. meningitidis. |
| Additional Information | 1) Rhamnose modified EF-P rescues ribosomes stalled at proline stretches in proteins. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Arg) linked |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | dTDP-l-Rhamnose |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Rha) |
| Method of Glycan Indentification | MALDI-TOF MS and ESI-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | EF-P |
| ProGPdb ID | ProGP521 |
| Litrature | |
| Year Of Validation | 2016 |
| Reference | Yanagisawa, T., Takahashi, H., Suzuki, T., Masuda, A., Dohmae, N. and Yokoyama, S., 2016. Neisseria meningitidis translation elongation factor P and its active-site arginine residue are essential for cell viability. PloS one, 11(2), p.e0147907. |
| Corresponding Author | RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan |