ProGT114 (Gtf2)
ProGT ID | ProGT114 (Gtf2) |
Organism Information | |
Organism Name | Streptococcus gordonii |
Clinical Implication | Pathogenic |
Domain | Bacteria |
Phylum | Firmicutes |
Classification | Family: Streptococcaceae Order: Lactobacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" |
Taxonomic ID (NCBI) | 1302 |
Genome Information | |
Gene Bank | AY028381 |
EMBL | AY028381 |
Gene Information | |
Gene Name | gtf2 |
Protein information | |
Protein Name | Gtf2Â |
UniProtKB/ SwissProt ID | Q79T00 |
UniProtKB Sequence | >sp|Q79T00|GTF2_STRGN Glycosyltransferase-stabilizing protein Gtf2 OS=Streptococcus gordonii GN=gtf2 PE=1 SV=1 MIQLFDYYNQETQDLHDSLLAAGYACPTIVIEANGFLPDDMISPYTYFLGDEEGVDHPLF FNQVPVPPFWEITGDHQVARVSDMGEERARIHYASQARGRLVKQVDWLDKKGQLRLSERY NKQGRCFAKTAYKSGQEAFNTTYYSTDGQERIVENHVTGDIILTLDQEPLRIFKSRVDFI RFFLERLDLDLDHILFNSLAYSFLVSHSLTGRAGQDILFWQEPLYDELPGNMQLILDNSQ LRTQTIVIPDLATYEKAMSLAAADQQQKFLHLGYHYDFKRDNYLRKDALILTHSDQIEGL DTLVQSLPQLVFRIAALTEMSPKLLSMLSYKNVVLYQNASLKQIEQLYLESDIYLDINHG GQVLQAVRKAFENNLLILGFEQTLHDRHYIAQQHIFDSSQPAQLASILEEALCGVEQMRS ALQAQGRHANDVPVSLYQETLQSLLGGQHG |
EMBL CDS | AAS86345.1 |
Sequence length | 450 AA |
Subcellular Location | Membrane (Integral component of membrane) |
Function in Native Organism | 1) Deletion of GtfB abolishes adhesin secretion in Streptococcus gordonii. |
Additional Information | 1) GtfB provides the primary binding site for adhesin and then recognize Ser/Thr residues already modified with GlcNAc. |
PDB ID | 5E9T 5E9U |
Glycosyltransferase Information | |
Glycosylation Type | O- (Ser/Thr) linked |
CAZY Family | GT8 |
EC Number (BRENDA) | 2.4.1.- |
Mechanism of Glycan Transfer | Sequential |
Donor Type | Nucleotide activated sugars |
Donor Specificity | UDP-GlcNAc |
Glycan Information | |
Glycan transferred | Monosaccharide (GlcNAc)Â |
Experimental_strategies | In vivo and In vitro |
Acceptor Subtrate Information | |
Acceptor Substrate name | GspB |
ProGPdb ID | ProGP229 |
Litrature | |
Year Of Validation | 2016Â |
Reference | Chen, Y., Seepersaud, R., Bensing, B. A., Sullam, P. M., & Rapoport, T. A. (2016). Mechanism of a cytosolic O-glycosyltransferase essential for the synthesis of a bacterial adhesion protein. Proceedings of the National Academy of Sciences, 113(9), E1190-E1199. |
Authors | Chen, Y., Seepersaud, R., Bensing, B. A., Sullam, P. M., & Rapoport, T. A. |
Research groups | 1 Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115; Department of Cell Biology, Harvard Medical School, Boston
2 San Francisco Veteran Affairs Medical Center, San Francisco, CA 94121; University of California, San Francisco
|
Corresponding Author | Rapoport, T. A. |
Contacts | 1 Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115. 2 Department of Cell Biology, Harvard Medical School, Boston. |