ProGT118 (EarP)
ProGT ID | ProGT118 (EarP) |
Organism Information | |
Organism Name | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) |
Clinical Implication | Pathogenic |
Domain | Bacteria |
Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Pseudomonadales Family : Pseudomonadaceae Genus : Pseudomonas Species : putida Strain : ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 |
Taxonomic ID (NCBI) | 160488 |
Genome Information | |
Gene Bank | AE015451 |
EMBL | AE015451 |
Gene Information | |
Gene Name | earP |
NCBI Gene ID | 1043242 |
Protein information | |
Protein Name | EF-P arginine 32 rhamnosyl-transferase |
UniProtKB/ SwissProt ID | Q88LS1 |
NCBI Ref Seq | WP_010952888.1 |
UniProtKB Sequence | >tr|Q88LS1|Q88LS1_PSEPK EF-P arginine 32 rhamnosyl-transferase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=earP PE=1 SV=1 MKATWDIFCSVVDNYGDIGVTWRLARQLVAEHGLAVRLWVDDLNAFTPMCPGADATAAQQ WQHGVDVRHWPAAWLPVAPADVVIGAFACQLPAAYVEAMRARPQPPLWLNLEYLSAEDWV EGCHGLPSPQPNGLRKVFFFPGFTDKTGGLLREGSLLARRDGFQQSAEARRAFLQGLGVD LVPGALLISLFAYENPQLGNWLDALATADQPCHLLVPQGRVVAGLSQWLGEGPLHVGDVR TRGALTVQVLPFVSQDDFDRLLWSCDFNAVRGEDSFVRAQWAGQPMLWHIYVQDENAHWE KLEAFLAHYRCGLSDDADAALLGLWRAWNMDFDMGQAWRAARQHWPELQQHARLWGARQA AQPDLATALVHFYRNSL |
EMBL CDS | AAN67476 |
Sequence length | 377 AA |
String | 160488.PP_1857 |
Potential Application | 1) The structures of EarP in complex with substrates should provide valuable information for the structure-guided development of its inhibitors such as EarP-containing pathogens specific antibacterials. |
Additional Information | 1) EarP is an EF-P arginine rhamnosyltransferase which is essential for post-translational activation. 2) EF-P and the corresponding rhamnosyltransferase EarP contributes to pathogenicity in Pseudomonas putida. 3) KOW-like EF-P N-domain is sufficient for EarP-mediated rhamnosylation. |
Glycosyltransferase Information | |
Glycosylation Type | N- (Arg) linked |
CAZY Family | GT104 |
Mechanism of Glycan Transfer | Sequential |
Donor Type | dTDP-l-Rhamnose |
Donor Specificity | Nucleotide activated sugars |
Glycan Information | |
Glycan transferred | Monosaccharide (Rha) |
Experimental_strategies | In vivo and In vitro |
Acceptor Subtrate Information | |
Acceptor Substrate name | EF-P |
ProGPdb ID | ProGP686 |
Litrature | |
Year Of Validation | 2017 |
Reference | Krafczyk, R., Macošek, J., Jagtap, P.K.A., Gast, D., Wunder, S., Mitra, P., Jha, A.K., Rohr, J., Hoffmann-Röder, A., Jung, K. and Hennig, J., 2017. Structural basis for EarP-mediated arginine glycosylation of translation elongation factor EF-P. MBio, 8(5), pp.e01412-17. |
Corresponding Author | 1 Center for integrated Protein Science Munich (CiPSM), Department of Biology I, Microbiology, Ludwig-Maximilians-Universität München, Munich, Germany.
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