ProGT12 (Rv1002c)

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ProGT ID ProGT12 (Rv1002c)
ProGT Pathway
Organism Information
Organism NameMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Clinical ImplicationPathogenic
DomainBacteria
Classification Phylum : Actinobacteria
Class : Actinomycetia
Orders : Corynebacteriales
Family : Mycobacteriaceae
Genus : Mycobacterium
Species : tuberculosis
Strain : ATCC 25618 / H37Rv
Taxonomic ID (NCBI)83332
Genome Information
Gene BankAL123456
EMBLAL123456
Gene Information
Gene Namepmt
NCBI Gene ID887882
Protein information
Protein NameRv1002c 
UniProtKB/ SwissProt IDP9WN05
NCBI Ref SeqNP_215518.1
UniProtKB Sequence>sp|P9WN05|PMT_MYCTU Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=pmt PE=1 SV=2 MTARPPESCVLAKDRPEEPVVPVVSPGPLVPVADFGPLDRLRGWIVTGLITLLATVTRFL NLGSLTDAGTPIFDEKHYAPQAWQVLNNHGVEDNPGYGLVVHPPVGKQLIAIGEAIFGYN GFGWRFTGALLGVVLVALVVRIVRRISRSTLVGAIAGVLLICDGVSFVTARTALLDGFLT FFVVAAFGALIVDRDQVRERMHIALLAGRSAATVWGPRVGVRWWRFGAGVLLGLACATKW SGVYFVLFFGAMALAFDVAARRQYQVQRPWLGTVRRDVLPSGYALGLIPFAVYLATYAPW FASETAIDRHAVGQAVGRNSVVPLPDAVRSLWHYTAKAFHFHAGLTNSAGNYHPWESKPW TWPMSLRPVLYAIDQQDVAGCGAQSCVKAEMLVGTPAMWWLAVPVLAYAGWRMFVRRDWR YAVVLVGYCAGWLPWFADIDRQMYFFYAATMAPFLVMGISLVLGDILYHPGQGSERRTLG LIVVCCYVALVVTNFAWLYPVLTGLPISQQTWNLEIWLPSWR
EMBL CDSCCP43752.1
Sequence length503 AA
Subcellular LocationMembrane
Function in Native Organism 1) Rv1002c is O-mannosyltransferase transfer the mannose to the alanine proline-rich protein Apa.
String83332.Rv1002c. 
Potential Application1) O-mannosylated and other glycoproteins are present on the cell surface of Mycbacterium tuberculosis and are involved in interaction with host cell during infection, this protein glycosyltransferase may be useful in designing of novel drug targets.
Additional Information1) Mycobacterial protein O-mannosylation occurs by a similar mechanism to that in eukaryotes.
Glycosyltransferase Information
Glycosylation TypeO- (Ser/Thr) linked 
CAZY FamilyGT39
EC Number (BRENDA)2.4.1.-
Donor TypePolyprenol phosphate-Mannose
Donor SpecificityLipid linked sugars
Accessory GT IDProGT12.1
Glycan Information
Glycan transferredMonosaccharide (Man) 
Method of Glycan IndentificationLC-ESI-MS/MS
Acceptor Subtrate Information
Acceptor Substrate name Apa
ProGPdb ID ProGP51
Acceptor Substrate name SodC
ProGPdb ID ProGP189
Litrature
Year Of Validation2005 
Reference VanderVen, B.C., Harder, J.D., Crick, D.C. and Belisle, J.T., 2005. Export-mediated assembly of mycobacterial glycoproteins parallels eukaryotic pathways. Science, 309(5736), pp.941-943.

Corresponding AuthorMycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA.
Reference Liu, C.F., Tonini, L., Malaga, W., Beau, M., Stella, A., Bouyssié, D., Jackson, M.C., Nigou, J., Puzo, G., Guilhot, C. and Burlet-Schiltz, O., 2013. Bacterial protein-O-mannosylating enzyme is crucial for virulence of Mycobacterium tuberculosis. Proceedings of the National Academy of Sciences, 110(16), pp.6560-6565.

Corresponding AuthorScientific Research National Center, Institute of Pharmacology and Structural Biology, F-31077 Toulouse, France.
Reference Córdova-Dávalos, L.E., Espitia, C., González-Cerón, G., Arreguín-Espinosa, R., Soberón-Chávez, G. and Servín-González, L., 2014. Lipoprotein N-acyl transferase (Lnt1) is dispensable for protein O-mannosylation by Streptomyces coelicolor. FEMS microbiology letters, 350(1), pp.72-82.

Corresponding AuthorDepartment of Molecular Biology and Biotechnology, Institute of Biomedical Research, National Autonomous University of Mexico, Ciudad Universitaria, Mexico City, Mexico City.