ProGT122 (AaNGT)
| ProGT ID | ProGT122 (AaNGT) |
| Organism Information | |
| Organism Name | Aggregatibacter aphrophilus |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Pasteurellales Family : Pasteurellaceae Genus : Aggregatibacter Species : aphrophilus |
| Taxonomic ID (NCBI) | 732 |
| Genome Information | |
| Gene Information | |
| Protein information | |
| Protein Name | AaNGT |
| Additional Information | 1) AaNGT is the first identified natural glycosyltransferase able to transfer GlcN moiety onto asparagine residues. 2) AaNGT also exhibited a different position-specific residue preference of substrate peptides from the NGT of Actinobacillus pleuropneumoniae (ApNGT). |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT41 |
| Mechanism of Glycan Transfer | Sequential |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser/Thr |
| Donor Type | UDP-Glc (Most preferred), UDP-Gal, UDP-Xyl, GDP-Glc, dGDP-Glc and UDP-GlcN |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharides (Glc, Gal, Xyl, and GlcN) |
| Method of Glycan Indentification | LC/MS-IT-TOF |
| Experimental_strategies | In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | TAMRA-DANYTK |
| Acceptor Substrate name | TLDDNGTMLFFK (human Hemopexin) |
| Litrature | |
| Year Of Validation | 2018 |
| Reference | Kong, Y., Li, J., Hu, X., Wang, Y., Meng, Q., Gu, G., Wang, P.G. and Chen, M., 2018. N-Glycosyltransferase from Aggregatibacter aphrophilus synthesizes glycopeptides with relaxed nucleotide-activated sugar donor selectivity. Carbohydrate research, 462, pp.7-12. |
| Corresponding Author | 1 The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center and School of Life Sciences, Shandong University, Jinan, Shandong 250100, China. |