ProGT13 (AglB)
| ProGT ID | ProGT13 (AglB) |
| Organism Information | |
| Organism Name | Methanococcus maripaludis (strain S2 / LL) |
| Clinical Implication | Non-pathogenic |
| Domain | Archaebacteria |
| Classification | Phylum : Euryarchaeota Class : Methanococci Orders : Methanococcales Family : Methanococcaceae Genus : Methanococcus Species : maripaludis Strain : S2 / LL |
| Taxonomic ID (NCBI) | 267377 |
| Genome Information | |
| Gene Bank | BX950229 |
| EMBL | BX950229 |
| Gene Information | |
| Gene Name | MMP1424 |
| NCBI Gene ID | 2761329 |
| Protein information | |
| Protein Name | AglB |
| UniProtKB/ SwissProt ID | Q6LXC8 |
| NCBI Ref Seq | WP_011171368.1 |
| UniProtKB Sequence | >tr|Q6LXC8|Q6LXC8_METMP Oligosaccharyl transferase, STT3 subunit OS=Methanococcus maripaludis (strain S2 / LL) GN=MMP1424 PE=4 SV=1 MGEFLNKVSDFFKKNEKIKIILILLFIGMMSFQIRAQTADMAFTDNSYLQDMFSDDNGRM YLTALDPYYYLRMTENYVNNDYSNVGETTVGIDGENIPYDTIQYAPPGREAGLVSALSIA TVLVYSVWNSIDSTVTIMNAAFWVPAIMSIFLGIPVFFIVRRNTASNIGGLVGALLLISS PSLLYKTSAGFSDTPIFEILPLLFIVWMIMEAIHEQENSKKSGIFGGIAAILIGLYPMMW SGWWYAFDITAGFLVLYTAYEYLTKSKNLKNVITTSLITLVGGAILVSLSTGLSGFINWI LSPIGFTVINEATKITGWPNVYMTVSELAIPTVTDIIENSVGNIWLLIAGISGILLSFVS FKHDKQKIDIKYALYLTLWLIATVYAATKGIRFVALMTPALAIGIGIFAGQIENIIKRYE KKVEYILYPVIGILSVITLIKYGGELFNILVPTTYVPIAVYLSIIALLVLAVYKIIDIIS EKEQAVKKVFGILLAFMLVFPSMAAAVPFYTAPTMNNGWMDSLSWIKSETPENSVVTCWW DNGHIYTWATRKMVTFDGGSQNTPRAYWVGHAFSTSDENLSVGILRMLATSGDSAYDDDS ILIKKTGSIKDTVDILNKILPLTRTEAKASLVNNYDLTDAEAEEVLDLTHPKVTNPDYLI TYNRMTSIASVWSMFGNWNFSLPASTENSDREMGYYQQLGGSAQDINGTTVVYIPLQETD SYRVINILEITDSEIKSANAVIDSNNQTSMQSPNFHKLILKVNGNVYEQETNENGDYSEI VRLEKLSDGTYQVYAWVSSKNLEDSIYTKLHFLDGYGLEKISLEKESVDPTSYGIQPGFK VYSVDYGTDYLN |
| EMBL CDS | CAF30980.1 |
| Sequence length | 852 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) AglB is OSTase transfer the tetrasaccharide to the S-layer protein. |
| String | 267377.MMP1424. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18. 11922. |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | DolP-Tetrasaccharide |
| Donor Specificity | Lipid linked sugars |
| Accessory GT ID | ProGT13.1, ProGT13.2, ProGT13.3, ProGT13.4, ProGT13.5, ProGT13.6, ProGT13.7, ProGT13.8, ProGT13.9, ProGT13.10, ProGT13.11, ProGT13.12, ProGT13.13, ProGT13.14, ProGT13.15, ProGT13.16, ProGT13.17, ProGT13.18, ProGT13.19 |
| Glycan Information | |
| Glycan transferred | Tetrasaccharide (N-acetylgalactosamine, di-acetylated glucuronic acid, an acetylated and acetamidino-modified mannuronic acid linked to threonine, and a novel terminal sugar [(5S)-2-acetamido-2,4-dideoxy-5-O-methyl-a-L-erythro-hexos-5-ulo-1,5-pyranose]) |
| Method of Glycan Indentification | Nano-LC-MS/MS |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | FlaB1 |
| ProGPdb ID | ProGP259 |
| Acceptor Substrate name | FlaB2 |
| ProGPdb ID | ProGP260 |
| Acceptor Substrate name | FlaB3 |
| ProGPdb ID | ProGP258 |
| Acceptor Substrate name | S-layer |
| Litrature | |
| Year Of Validation | 2009 |
| Reference | Siu, S., Robotham, A., Logan, S.M., Kelly, J.F., Uchida, K., Aizawa, S.I. and Jarrell, K.F., 2015. Evidence that biosynthesis of the second and third sugars of the archaellin tetrasaccharide in the archaeon Methanococcus maripaludis occurs by the same pathway used by Pseudomonas aeruginosa to make a di-N-acetylated sugar. Journal of bacteriology, 197(9), pp.1668-1680. |
| Corresponding Author | Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada |
| Reference | VanDyke, D.J., Wu, J., Logan, S.M., Kelly, J.F., Mizuno, S., Aizawa, S.I. and Jarrell, K.F., 2009. Identification of genes involved in the assembly and attachment of a novel flagellin N?linked tetrasaccharide important for motility in the archaeon Methanococcus maripaludis. Molecular microbiology, 72(3), pp.633-644. |
| Corresponding Author | Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada |
| Reference | Holten, M.P., Fonseca, D.R. and Costa, K.C., 2021. The Oligosaccharyltransferase AglB Supports Surface-Associated Growth and Iron Oxidation in Methanococcus maripaludis. Applied and environmental microbiology, 87(17), pp.e00995-21. |
| Corresponding Author | Department of Plant and Microbial Biology, University of Minnesota, Twin Cities, St. Paul, Minnesota, USA |