ProGT134 (PalS)
| ProGT ID | ProGT134 (PalS) |
| Organism Information | |
| Organism Name | Aeribacillus pallidus 8 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Bacillaceae Genus : Aeribacillus Species : pallidus Strain : 8 |
| Taxonomic ID (NCBI) | 33936 |
| Genome Information | |
| Gene Bank | LVHY01000134.1 |
| Gene Information | |
| Gene Name | palS |
| Protein information | |
| Protein Name | PalS |
| Function in Native Organism | Found in glycocin biosynthesis cluster and transfer the sugar to the glycocin, which is required for the antimicrobial activity |
| Additional Information | Also used as a glycoengineering tool to transfer the sugar glucose to the glycocins (Hyp1 and Hyp2) present in glycocin biosynthesis clusters of other bacteria Bacillus megaterium BHG1 Bacillus sp. JCM19047 |
| Glycosyltransferase Information | |
| Glycosylation Type | S- (Cys) linked |
| CAZY Family | GT2 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-Glc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharides (Glc) |
| Method of Glycan Indentification | LC-ESI-QMS/MS mass spectrometery |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | PalA |
| ProGPdb ID | ProGP703 (PalA) |
| Acceptor Substrate name | Hyp1 |
| ProGPdb ID | ProGP699 (Hyp1) |
| Acceptor Substrate name | Hyp2 |
| ProGPdb ID | ProGP700 (Hyp2) |
| Litrature | |
| Year Of Validation | 2019 |
| Reference | Kaunietis, A., Buivydas, A., ?itavi?ius, D.J. and Kuipers, O.P., 2019. Heterologous biosynthesis and characterization of a glycocin from a thermophilic bacterium. Nature communications, 10(1), pp.1-12. |
| Corresponding Author | 1. Molecular Genetics Dept., Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, Netherlands |