ProGT143 (SvGT)
ProGT ID | ProGT143 (SvGT) |
Organism Information | |
Organism Name | Streptomycetes venezulae strain 15439 |
Clinical Implication | Non-pathogenic |
Domain | Bacteria |
Classification | Phylum : Actinobacteria Class : Actinomycetia Orders : Streptomycetales Family : Streptomyycetaceae Genus : Streptomyces Species : venezuelae |
Taxonomic ID (NCBI) | 54571 |
Genome Information | |
Gene Bank | CP013129.1 |
Gene Information | |
Gene Name | SvGT |
Protein information | |
Protein Name | SvGT |
NCBI Ref Seq | ALO08693.1 |
Function in Native Organism | SvGT define the distinct donor specificity, acceptor specificity, regioselectivity, chemoselectivity, and Y(G/A/K/Q/E??G)(C/S/T?Y/N)(G/A?P/Q)G as the minimum acceptor sequon of SvGT. Although UDP-GlcNAc served as the donor in the cellular milieu, SvGT could also utilize UDP-Glc and UDP-GalNAc as donors in vitro |
Additional Information | Also used as glycoengineering tool for modification of neo glycocin |
Glycosyltransferase Information | |
Glycosylation Type | S- (Cys) and O- (Ser/Thr) linked |
CAZY Family | GT2 |
Mechanism of Glycan Transfer | Sequential |
Donor Type | UDP-GlcNAc, UDP-Glc, UDP-GalNAc |
Donor Specificity | Nucleotide activated sugars |
Glycan Information | |
Glycan transferred | Monosaccharide (GlcNAc, Glc, GalNAc) |
Method of Glycan Indentification | mass spectrometry and western blotting |
Experimental_strategies | In vivo and In vitro |
Acceptor Subtrate Information | |
Acceptor Substrate name | SvC |
ProGPdb ID | ProGP1215 (SvC) |
Litrature | |
Year Of Validation | 2021 |
Reference | Sharma, Y., Ahlawat, S. and Rao, A., 2021. Biochemical characterization of an inverting S/O-HexNAc-transferase and evidence of S-linked glycosylation in Actinobacteria. Glycobiology. |
Corresponding Author | 2 CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India |