ProGT26 (TfpW)
| ProGT ID | ProGT26 (TfpW) |
| Organism Information | |
| Organism Name | Pseudomonas aeruginosa Pa5196 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Pseudomonadales Family : Pseudomonadaceae Genus : Pseudomonas Species : aeruginosa Strain : PA7 |
| Taxonomic ID (NCBI) | 381754 |
| Genome Information | |
| Gene Bank | AY112720 |
| EMBL | AY112720 |
| Gene Information | |
| Protein information | |
| Protein Name | TfpW |
| UniProtKB/ SwissProt ID | Q8KQ31 |
| NCBI Ref Seq | AAM52060.1 |
| UniProtKB Sequence | >tr|Q8KQ31|Q8KQ31_PSEAI Putative arabinosyltransferase TfpW OS=Pseudomonas aeruginosa PE=4 SV=1 MKSLWVYLQGGGKKNVLLWVGVLCAVVWGLYGRALFFGYVWDDSILFLDKTDLLNSSISW KVLSEPVLPGTSYLRPLVFLSFFVEFQIFGQSPFVSHAVNIAIFNLNVLLVFWLGSTLAR HTERKYGLLLAFLAALFYAMHPALIESTVWASGRFDLLTTFFILAGLVVYLADIKSTVLR AFLLCLCMAGALLSKELGIVFPVLLLCLWLAKECDCSTQRGGALLVQALFRNGWMLVALI ITAFGYLVVRKHSAGGIYHSPVGLSYIADVVLGQRLPLETLKFYLLQSFLPFQSINVSHP LNEVFERSVYVLLAGQMVVLILVLTVIYQALYRRSAAAWMFFAYLVCLLPVLHIIPLTIG ENLGHERFLTAPLAFLALALVFVRYDRVLSKVGLSGAPLLRVGLVAGLLWLGMAGFTIYS ILPMWTSDASLWAWAYKTHPESKYARYDYLYGALRGGYVELLDKEVSRLQKKYGGLEVPE QILYAHRLISAGDSEGLNYLEGVLQVLPRFHDAENGRVALNDFKHLSSMQIGGAYAVYAE GLSMLRGDAERALKYNKIAEWYLATGEVIPLMYQRSAILYALGDYGQAARIYQDQEGLHY YMSQAVKKDMKRLVEGFCKEKGFPSDPCGELRERGVISKESEARQG |
| Sequence length | 646 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) TfpW transfer a D-Araf of a trisaccharide to the pilin protein. |
| Potential Application | 1) Study of D-Araf biosynthesis pathways may help in the identification of the new class of antibiotics against Corynebacterineae. |
| Additional Information | 1) TfpW is the first reported gram-negative member of the GT-C glycosyltransferase family which is involved in type IV pilin glycosylation. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GTNC |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | En bloc |
| Glycan Information | |
| Glycan transferred | Alpha1,5-D-Araf |
| Method of Glycan Indentification | ETD-MS, and ESI-MS |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | PilA |
| ProGPdb ID | ProGP230 |
| Litrature | |
| Year Of Validation | 2008 |
| Reference | Kus, J.V., Kelly, J., Tessier, L., Harvey, H., Cvitkovitch, D.G. and Burrows, L.L., 2008. Modification of Pseudomonas aeruginosa Pa5196 type IV pilins at multiple sites with d-Ara f by a novel GT-C family arabinosyltransferase, TfpW. Journal of bacteriology, 190(22), pp.7464-7478. |
| Corresponding Author | Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada |
| Reference | Harvey, H., Kus, J.V., Tessier, L., Kelly, J. and Burrows, L.L., 2011. Pseudomonas aeruginosa D-arabinofuranose biosynthetic pathway and its role in type IV pilus assembly. Journal of Biological Chemistry, 286(32), pp.28128-28137. |
| Corresponding Author | Michael G DeGroote Institute for Infectious Diseases Research and the Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada. |