ProGT38 (Gtf2)
| ProGT ID | ProGT38 (Gtf2) |
| Organism Information | |
| Organism Name | Streptococcus parasanguinis |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Lactobacillales Family : Streptococcaceae Genus : Streptococcus Species : parasanguinis |
| Taxonomic ID (NCBI) | 1318 |
| Genome Information | |
| Gene Bank | DQ990875.1 |
| EMBL | DQ990875 |
| Gene Information | |
| Gene Name | gtf2 |
| NCBI Reference Sequence | ABL74005.1 |
| Protein information | |
| Protein Name | Gtf2 |
| UniProtKB/ SwissProt ID | A1C3M0 |
| NCBI Ref Seq | WP_014713988.1 |
| UniProtKB Sequence | >sp|A1C3M0|GTF2_STRPA Glycosyltransferase-stabilizing protein Gtf2 OS=Streptococcus parasanguinis GN=gtf2 PE=1 SV=1 MIRLFEWLTQESLDLHYSLEESGIHGTSIVLNDDGFLPEGIISPYTFFCEVEMDGSPLYF NQLEVPYLWQITGTNIEGEIWNRSSKRGVIHYHEPKYLRFVQSVDWLYPDGSIYMTDHYN KYGWAFARTYFFSDQQVSHKKYYTKSGQEVLSENILTGDILLNWKGKVYHFTKKVDFFLF YFKKSGLDLSSIWYNSLGMPFLISYYLGGEGRDILFWQENLADQLPGNMQIIFSGRTSRT KKVIVQDRSVYKKLLHLVEEKNKEMISFLNIIYPKLRENYSRKEILIVTNSDQIEGIETL TDNLSAYTFHIGALTSMSDKLQNIGQKENVLLYPNMSPKTMLDLLEQCDIYLDINHGNEV LSIVRLAFERSLLILAYDNTVHSPIFHHESGIFNHSKPQTLSDWLLNLDDYSQTVSCWRS DLFPMTYRDYKQVLVSNVD |
| EMBL CDS | ABL74005.1. |
| Sequence length | 439 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) The complex of two GTs namely Gtf1 and Gtf2 initiates the glycosylation of by transferring the GlcNAc to Fap 1 protein. |
| Potential Application | 1) Studying biosynthetic pathways and the enzymes involved in biofilm formation may help to design of new potential therapeutics to control bacterial biofilm formation in future. |
| Additional Information | 1) The N-terminal domain of Gtf1 directly interacts with Gtf2 and this interaction plays a critical role in Fap1 glycosylation. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT8 |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc |
| Donor Specificity | Nucleotide activated sugars |
| Accessory GT ID | ProGT37.1,ProGT37.2 |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Method of Glycan Indentification | GC-MS |
| Experimental_strategies | In vitro and In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Fap1 |
| ProGPdb ID | ProGP209 |
| Litrature | |
| Year Of Validation | 2010 |
| Reference | Zhou, M., Zhu, F., Dong, S., Pritchard, D.G. and Wu, H., 2010. A novel glucosyltransferase is required for glycosylation of a serine-rich adhesin and biofilm formation by Streptococcus parasanguinis. Journal of Biological Chemistry, 285(16), pp.12140-12148. |
| Corresponding Author | Department of Pediatric Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35244, USA |
| Reference | Zhu, F., Zhang, H., Yang, T., Haslam, S.M., Dell, A. and Wu, H., 2016. Engineering and dissecting the glycosylation pathway of a streptococcal serine-rich repeat adhesin. Journal of Biological Chemistry, 291(53), pp.27354-27363. |
| Corresponding Author | University of Alabama at Birmingham, United States |