1) SunS is the S-glycosyltransferase characterized in vitro which glycosylates SunA peptide with one hexose. 2) SunS recognizes alpha-helix at the N-terminal of the substrate peptide and modifies the Cys residue present in the loop region of SunA. 3) SunS does not modify SunA-C22S mutant, therefore displays high chemoselectivity towards thiol group of a cysteine residue.
Howard Hughes Medical Institute and Roger Adams Laboratory, Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
