ProGT56 (PglB)

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ProGT ID ProGT56 (PglB)
ProGT Pathway
Organism Information
Organism NameCampylobacter Jejuni JCM 2013
Clinical ImplicationPathogenic
DomainBacteria
Classification Phylum : Proteobacteria
Class : EpsilonProteobacteria
Orders : Campylobacterales
Family : Campylobacteraceae
Genus : Campylobacter
Species : jejuni
Subspecies : jejuni
Strain : JCM 2013
Taxonomic ID (NCBI)1236539
Genome Information
Gene BankAB872218.1
EMBLAB872218 
Gene Information
Gene NamepglB
NCBI Reference SequenceAB872218 
Protein information
Protein NamePglB 
UniProtKB/ SwissProt IDA0A060NG81
NCBI Ref SeqWP_002879052.1
UniProtKB Sequence>tr|A0A060NG81|A0A060NG81_CAMJU Putative oligosaccharyltransferase OS=Campylobacter jejuni subsp. jejuni OX=32022 GN=pglB PE=4 SV=1 MLKKEYLKNPYLVLFAMIVLAYVFSVFCRFYWVWWASEFNEYFFNNQLMIISNDGYAFAE GARDMIAGFHQPNDLSYYGSSLSALTYWLYKVTPFSFESIILYMSTFLSSLVVIPIILLA NEYKRPLMGFVAALLASIANSYYNRTMSGYYDTDMLVIVLPMFILFFMVRMILKKDFFSL IALPLFIGIYLWWYPSSYTLNVALIGLFLIYTLIFHRKEKIFYIAVILSSLTLSNIAWFY QSAIIVILFALFALEQKRLNFMIIGILGSATLIFLILSGGVDPILYQLKFYIFRSDESAN LTQGFMYFNVNQTIQEVENVDFSEFMRRISGSEIVFLFSLFGFVWLLRKHKSMIMALPIL VLGFLALKGGLRFTIYSVPVMALGFGFLLSEFKAILVKKYSQLTSNVCIVFATILTLAPV FIHIYNYKAPTVFSQNEASLLNQLKNIANREDYVVTWWDYGYPVRYYSDVKTLVDGGKHL GKDNFFPSFALSKDEQAAANMARLSVEYTEKSFYAPQNDILKSDILQAMMKDYNQSNVDL FLASLSKPDFKIDTPKTRDIYLYMPARMSLIFSTVASFSFINLDTGVLDKPFTFSTAYPL DVKNGEIYLSNGVVLSDDFRSFKIGDNVVSVNSIVEINSIKQGEYKITPIDDKAQFYIFY LKDSAIPYAQFILMDKTMFNSAYVQMFFLGNYDKNLFDLVINSRDAKVFKLKI
EMBL CDSBAO79832.1
Sequence length713 AA
Subcellular LocationMembrane (Integral component of membrane)
Function in Native Organism
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
EC Number (BRENDA)2.4.1.119
Mechanism of Glycan TransferEn bloc
Acceptor specificity Sequon_1Asp/Glu- Asn-Xaa-Ser/Thr
Donor TypeUndPP-Heptasaccharide
Donor SpecificityLipid linked sugars
Accessory GT IDProGT56.1, ProGT56.2, ProGT56.3, ProGT56.4, ProGT56.5, ProGT56.6, ProGT56.7, ProGT56.8, ProGT56.9, ProGT56.10
Glycan Information
Glycan transferredHeptasaccharide (GalNAca-GalNAca-(Hex-)HexNAc-HexNAc-HexNAcHexNAc)  
Method of Glycan IndentificationNanoLC-MS/MS
Experimental_strategiesIn vivo 
Acceptor Subtrate Information
Acceptor Substrate name CmeA
ProGPdb ID ProGP217
Litrature
Year Of Validation2014 
Reference Srichaisupakit, A., Ohashi, T. and Fujiyama, K., 2014. Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli. Journal of bioscience and bioengineering, 118(3), pp.256-262.

Corresponding AuthorInternational Center for Biotechnology, Osaka University, Suita, Osaka 565-0871, Japan.