ProGT58 (TfpO)
| ProGT ID | ProGT58 (TfpO) |
| Organism Information | |
| Organism Name | Pseudomonas amygdali pv. tabaci (Pseudomonas syringae pv. tabaci 6605) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Pseudomonadales Family : Pseudomonadaceae Genus : Pseudomonas Species : amygdali |
| Taxonomic ID (NCBI) | 322 |
| Genome Information | |
| Gene Information | |
| Gene Name | tfpO |
| NCBI Reference Sequence | L14775 |
| Protein information | |
| Protein Name | TfpO |
| UniProtKB/ SwissProt ID | G5EJ36 |
| UniProtKB Sequence | >tr|G5EJ36|G5EJ36_PSEAJ Putative pilin glycosyltransferase OS=Pseudomonas amygdali pv. tabaci OX=322 GN=tfpO PE=4 SV=1 MIFFLYCCAVLIISSCFYDFSGHDYQRVAQFFIGLSSLFMLLKYSAKQLSFYTVSPQVKL FFFSVVVMGVVSSLMSHRPLWALAELSVLLLSCGISYAFALQRNFYGVRLDQLFMGFVVL ICSLKIIQFLGSAAAAFLSSAPILDTDLLLDGFSNKRFYGQFQTFTLPLLVLPLLLTSTR RSIKIGLFCLTSLWWMIAISGGTRGTWLGMGVAVALMFCLGHAGRRWSGWQLAAAMAGLL LFTLLFSVLPGLLGIEVSNFAGDRLTTSLSAREILWQQAWAMIKERPLLGFGPMHFADIW NAVAAHPHQAILQWACEWGIPSTLCVAGLALYGLSTTAVLVRKRALSLEPVDLMRLCLFA SLIGALTQSMVDGVIVMPYSQLWLAIIIGWLLALHEWQIAPRPASIALSRAWLLCLTLAT GMILYTIVRDVPDMDTRRQQFSEDFGGRYLPRFWMQGVIADPPAR |
| EMBL CDS | BAL05074.1 |
| Sequence length | 465 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) tfpO is required for pilin glycosylation in P. amygdali pv. tabaci 6605. |
| Additional Information | 1) Deletion of tfpO gene encoding TfpO shows a production of unglycosylated Pilin which indicates that it is required for pilin glycosylation. 2) TfpO mutant also shows a reduction in surface motility and virulence toward host tobacco plant which shows that pilin glycan plays an important role in bacterial motility and virulence. |
| Glycosyltransferase Information | |
| EC Number (BRENDA) | 2.4.99.18 |
| Glycan Information | |
| Method of Glycan Indentification | LC-ESI-MS |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | PilA |
| ProGPdb ID | ProGP230 |
| Litrature | |
| Year Of Validation | 2012 |
| Reference | Nguyen, L.C., Taguchi, F., Tran, Q.M., Naito, K., Yamamoto, M., OHNISHI?KAMEYAMA, M.A.Y.U.M.I., Ono, H., Yoshida, M., Chiku, K., Ishii, T. and Inagaki, Y., 2012. Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and is required for surface motility and virulence. Molecular plant pathology, 13(7), pp.764-774. |
| Corresponding Author | Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama 700-8530, Japan |