ProGT69 (GtfA)
| ProGT ID | ProGT69 (GtfA) |
| Organism Information | |
| Organism Name | Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) |
| Clinical Implication | Non-pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Lactobacillales Family : Lactobacillaceae Genus : Lactobacillus Species : plantarum Strain : ATCC BAA-793 / NCIMB 8826 / WCFS1 |
| Taxonomic ID (NCBI) | 220668 |
| Genome Information | |
| Gene Bank | AL935263.2 |
| EMBL | AL935263 |
| Gene Information | |
| Gene Name | tagE5 (lp_2843) |
| NCBI Gene ID | 1063213 |
| Protein information | |
| Protein Name | GtfA |
| UniProtKB/ SwissProt ID | F9URU6 |
| NCBI Ref Seq | YP_004890449 |
| UniProtKB Sequence | >tr|F9URU6|F9URU6_LACPL Poly(Glycerol-phosphate) alpha-glucosyltransferase OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) GN=tagE5 PE=4 SV=1 MNFFVNQYLMALNSGVEHAEFKRLQLFKDHQAPAKLVTRQFDGLLHQNMRRFNIADDQMA NLFDFFRETTSFSSRVIKMADLNWPTAYDIKPDPNVSEVRAGDRLVAKVHFVPSTVGHVY FIEIFDHFNNLVQRTDYDERGFKARDEFFSSAGEPITDIFYRPDGSRMAEQYYTHDNNGT NYVSLCHLINYHGRDYYFNGEQEWYRFFLDELNKQYDENNVFIADRPLAAQWPVVNMQTA AKKYLWLPTPHAVDPRDQVYSNLNNAYVYGLHEFLSAWDGVITSTEQQKQDLTQWLGGQP SVPILTISAAIVSKQQAQHAPISISERHAHEIIYTGRLDAERRVDQLVTAFDQVHHKITD ATLVIYGYGGELDRLKQQVSQLHLETAVTFAGYQPNLTMAYDQAGAYVYTGESDAQPLSL IEALSHGVPAIAYDINYGPKDVLKDGKNGYLVTDGAVNQLVRALTRTLESPQRQQRLSKG AYRSSEQYSDEQVWKQWQPVVQ |
| EMBL CDS | CCC79935.1 |
| Sequence length | 502 AA |
| Function in Native Organism | 1) Deletion studies of Six TagE show that both Both TagE5 and TagE6 are required for protein glycosylation in L. plantarum WCFS1. |
| String | 220668.lp_2843 |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT4 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Method of Glycan Indentification | MALDI-TOF MS/MS and LC-MS/MS |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Acm2 |
| ProGPdb ID | ProGP436 |
| Litrature | |
| Year Of Validation | 2014 |
| Reference | Lee, I.C., van Swam, I.I., Tomita, S., Morsomme, P., Rolain, T., Hols, P., Kleerebezem, M. and Bron, P.A., 2014. GtfA and GtfB are both required for protein O-glycosylation in Lactobacillus plantarum. Journal of bacteriology, 196(9), pp.1671-1682. |
| Corresponding Author | TI Food and Nutrition, Wageningen, the Netherlands. |