ProGT79 (BAHTCr)
| ProGT ID | ProGT79 (BAHTCr) |
| Organism Information | |
| Organism Name | Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Enterobacterales Family : Enteerobacteriaceae Genus : Citrobacter Species : rodentium Strain : ICC168 |
| Taxonomic ID (NCBI) | 637910 |
| Genome Information | |
| Gene Bank | FN543503 |
| EMBL | FN543503 |
| Gene Information | |
| Gene Name | ROD_p1111 |
| NCBI Reference Sequence | FN543503 |
| Protein information | |
| Protein Name | BAHTCr |
| UniProtKB/ SwissProt ID | D2TV87 |
| NCBI Ref Seq | WP_012908907.1 |
| UniProtKB Sequence | >tr|D2TV87|D2TV87_CITRI Putative adhesin glycosyltransferase OS=Citrobacter rodentium (strain ICC168) GN=ROD_p1111 PE=4 SV=1 MQKSLATFFISPPEIPTQHGPDNILYDFNDGARVLLPEGKWHVRLLDADSGNILFCCDIN NGWVTSSKKYFVRFRIQVFRQGEDSPLLDETLNLTDRDVLISFPTGTLGDLLGWFPYAER FQSLHQCRLECTMAQDIIDLLAPQYPQICFSTPEKPRTTEPYATYRVGLYFGGDTNNQPV DFRQVGFHRSAGYILGVDPREAPVRLNLSAPCTIREPYVCIATQSTCQAKYWNNGTGWSE VVAHLKSLGYRVLCIDREAHYGQGFVWNHIPWGAEDFTGSFPLQERVNLLRHASFFIGLA SGLSWLAWATGIPVVLISGFSLPDSEFYTPWRVFNSHGCNGCWDDTSLNFDHKDFLWCPR HKNTDRQFECTRLITGTQVNGVISRLHASLMKQGDKACLTKGTNNEQGL |
| EMBL CDS | CBG91773.1 |
| Sequence length | 409 AA |
| Additional Information | 1) BAHTCr, heptosyltransferases able complement AAH (Escherichia coli) in restoring AIDA-I glycosylation and bacterial adhesion to HeLa cells. 2) BAHTCr is involved in a transfer of heptose to CARC autotransporter which is very critical for C. rodentium colonization in mice colon. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GTNC |
| EC Number (BRENDA) | 2.4.1.- |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | ADP-Heptose |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Heptose) |
| Method of Glycan Indentification | ECL glycoprotein detection kit |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | AIDA-I (CARC) |
| ProGPdb ID | ProGP201 |
| Acceptor Substrate name | TibA |
| ProGPdb ID | ProGP176 |
| Litrature | |
| Year Of Validation | 2014 |
| Reference | Lu, Q., Yao, Q., Xu, Y., Li, L., Li, S., Liu, Y., Gao, W., Niu, M., Sharon, M., Ben-Nissan, G. and Zamyatina, A., 2014. An iron-containing dodecameric heptosyltransferase family modifies bacterial autotransporters in pathogenesis. Cell host & microbe, 16(3), pp.351-363. |
| Corresponding Author | 1 National Institute of Biological Sciences, Beijing 102206, China. 2 National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, 3 China National Institute of Biological Sciences, Beijing, Collaborative Innovation Center for Cancer Medicine, Beijing 102206, China. |
| Reference | Yao, Q., Lu, Q., Wan, X., Song, F., Xu, Y., Hu, M., Zamyatina, A., Liu, X., Huang, N., Zhu, P. and Shao, F., 2014. A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family. Elife, 3, p.e03714. |
| Corresponding Author | Dr Feng Shao's Laboratory, National Institute of Biological Sciences, Beijing, China |