ProGT97 (DvPglB)
| ProGT ID | ProGT97 (DvPglB) |
| Organism Information | |
| Organism Name | Desulfovibrio vulgaris subsp. vulgaris str. Hildenborough |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : DeltaProteobacteria Orders : Desulfovibrionales Family : Desulfovibrionaceae Genus : Desulfovibrio Species : vulgaris Strain : Hildenborough |
| Taxonomic ID (NCBI) | 882 |
| Genome Information | |
| Gene Bank | AE017285 |
| EMBL | AE017285 |
| Gene Information | |
| Gene Name | DVU_1252 |
| Protein information | |
| Protein Name | DvPglB |
| UniProtKB/ SwissProt ID | Q72CN1 |
| NCBI Ref Seq | WP_010938548.1 |
| UniProtKB Sequence | >tr|Q72CN1|Q72CN1_DESVH Membrane protein, putative OS=Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303) GN=DVU_1252 PE=4 SV=1 MTRLRGTLSVCIDAFPAWTRPLALAFVVYALSFGMRMLEYPAWQNPEYMLDGEYLLATHD AYHWIAGAEGFEFGAGHPMSELVRLAAAFTGTTPAAVGFWLPPVMASLVAVGVFGWAAAL GGVEAGVCAGVLASLAPGFLARTLLGYCDTDLVTLLFPLLMGLGPAFWMARFMFTPLQVA RRLLARRTGTSDVVPDAVAVMMTSRGEADDDWHGGALSPRWLALLAGSGLVGSWGAEWHS LFPYLIRYDVVLLFGCICVFGRPGMRRALLVGGLAYALPMLVGAWGLLFALALGWGLHRA DARFMAFVRGRALPVAGWCAVAVLLFDPDVFRMFLNSIAGYVKRSGDPSPGAGGDDPLVY PSVAQSIIEVQDLSLSEVLSYFHPWLWVALAGLGGFVPLLLARPAAFFLLPLGVLSFLSV KLGGRMVMFGAPVLALGFALPMVWGVQRVLRHDLRTGWVRLALSAVLLVIVATPFVDLLP AMTQGPIINKRHAEALRHIRTATPEDSMVWIWWDWGYSAHHFAHRRTIADGASHGGPSLY VPAAVFSTANPRFAWQLIRYTAERGGIPGSVFEGMGGAQAQELVHRLGIEKMTFADAPRQ YLVVSYDMLRLGFWITNFGTWDFLSREGKGYAISSIPQQLSYSLDTGEVQVQGNTTGVLA STIDVFDEGQLERRDYIVRQGGSGNAIADYAARQRNIESRRNIHFLFNKVTGEKLVLDDR LYNTVMVQLLLCSPDDTRFAPYFKLIYDNVYARVYEVR |
| EMBL CDS | AAS95730.1 |
| Sequence length | 758 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| String | 882.DVU1252 |
| Additional Information | 1) It is able to transfer glycans to the acceptor sites and has relaxed substrate specificity towards negatively charged residue in the ?2 position relative to the asparagine as compared to C. jejuni PglB. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | En bloc |
| Acceptor specificity Sequon_1 | Asn-Xaa-Ser |
| Donor Type | UndPP-Heptasaccharide |
| Donor Specificity | Lipid linked sugars |
| Glycan Information | |
| Glycan transferred | Heptasaccharide |
| Method of Glycan Indentification | LC-MS |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | scFv13-R4 LQNAT |
| Acceptor Substrate name | scFv13-R4 MQNAT |
| Acceptor Substrate name | scFv13-R4 QQNAT |
| Acceptor Substrate name | TAMRA-GDQNATAF |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. and DeLisa, M.P., 2015. Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5(1), pp.1-13. |
| Corresponding Author | Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853. |